State Key Laboratory of Ophthalmology, Optometry and Vision Science, School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou 325027, China; School of Chemical Engineering, Nanjing University of Science & Technology, Nanjing 210094, China.
School of Chemical Engineering, Nanjing University of Science & Technology, Nanjing 210094, China.
Int J Biol Macromol. 2020 Nov 15;163:366-374. doi: 10.1016/j.ijbiomac.2020.06.283. Epub 2020 Jul 3.
With the rapid development of biomedicine applications, more flexible and efficient fabrication strategies are required for drug delivery vehicles. Naturally resourced polymers have gained considerable attention for construction of drug carriers. Here, biocompatible hydrogel reservoir system (named PHG-PDA) based on pullulan and polydopamine (PDA) are designed and fabricated by one-pot incorporation of PDA fibers into pullulan hydrogel (PHG). The structure and performance of the PHG-PDAs can be nicely adjusted by regulating the content of PDA fibers in precursor hydrogel solutions. Crystal violet is used as a drug prototype and introduced into hydrogels via swelling-diffusion approach. We found that the drug loading process followed the pseudo-second-order model and Langmuir isotherm model, while the drug releasing process was pH-responsive, and the cumulative release reached 60.3% and 87% at pH 7.4 and 5.0, respectively. In vitro cytotoxicity studies on L929 cells demonstrated that our hydrogels were nontoxic even in a high concentration (3.4 mg/mL). In sum, these biocompatible PHG-PDAs with tunable physicochemical properties are promising systems for drug delivery.
随着生物医学应用的快速发展，需要更灵活、高效的药物输送载体制造策略。天然来源的聚合物因其可构建药物载体而受到广泛关注。在这里，我们设计并制备了基于普鲁兰多糖和聚多巴胺（PDA）的生物相容性水凝胶储库系统（命名为 PHG-PDA），方法是将 PDA 纤维一锅法掺入普鲁兰多糖水凝胶（PHG）中。通过调节前驱体水凝胶溶液中 PDA 纤维的含量，可以很好地调节 PHG-PDAs 的结构和性能。结晶紫被用作药物原型，并通过溶胀-扩散法引入水凝胶中。我们发现，药物负载过程遵循伪二阶模型和朗缪尔等温模型，而药物释放过程是 pH 响应的，在 pH 值为 7.4 和 5.0 时，累积释放率分别达到 60.3%和 87%。体外 L929 细胞毒性研究表明，即使在高浓度（3.4mg/mL）下，我们的水凝胶也没有毒性。总之，这些具有可调理化性质的生物相容性 PHG-PDAs 是很有前途的药物输送系统。
