Department of Integrated TCM & Western Medicine, Liaoning Cancer Hospital &Institute, Shenyang, Liaoning, 110043, China.
Department of Integrated TCM & Western Medicine, Liaoning Cancer Hospital &Institute, Shenyang, Liaoning, 110043, China.
Neurosci Lett. 2020 Sep 14;735:135153. doi: 10.1016/j.neulet.2020.135153. Epub 2020 Jul 3.
Circular RNAs (circRNAs) are related to the carcinogenesis of cancers, including glioma. However, the role and mechanism of circRNA pleiotrophin (circ-PTN) remain largely unknown.
Glioma tissues (n = 30) and normal tissues were obtained. Glioma cell lines LN229 and A172 were cultured for experiments in vitro. circ-PTN, microRNA-432-5p (miR-432-5p) and Ras-related protein Rab-10 (RAB10) levels were examined via quantitative reverse transcription polymerase chain reaction or western blot. Cell proliferation, invasion and glycolysis were examined via 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide, colony formation analysis, transwell invasion analysis, specific glucose, lactate or adenosine triphosphate assay kit and western blot. The relationship of miR-432-5p and circ-PTN or RAB10 was analyzed via dual-luciferase reporter analysis. The effect of circ-PTN on glioma development in vivo was explored by a murine xenograft model.
circ-PTN expression was enhanced and miR-432-5p abundance was reduced in glioma tissues and cells. circ-PTN silence suppressed cell proliferation, invasion and glycolysis. circ-PTN regulated glioma development by directly sponging miR-432-5p. RAB10 was a target of miR-432-5p and miR-432-5p inhibited cell proliferation, invasion and glycolysis by targeting RAB10. circ-PTN could modulate RAB10 expression via miR-432-5p. circ-PTN knockdown reduced glioma cell xenograft tumor growth in vivo.
circ-PTN knockdown repressed cell proliferation, invasion and glycolysis in glioma via modulating miR-432-5p and RAB10.
环状 RNA（circRNAs）与癌症的发生有关，包括神经胶质瘤。然而，circRNA 多效蛋白（circ-PTN）的作用和机制在很大程度上仍然未知。
收集 30 例神经胶质瘤组织和正常组织。体外培养神经胶质瘤细胞系 LN229 和 A172 进行实验。通过实时定量逆转录聚合酶链反应或 Western blot 检测 circ-PTN、microRNA-432-5p（miR-432-5p）和 Ras 相关蛋白 Rab-10（RAB10）的水平。通过 3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2-H-四唑溴盐、集落形成分析、Transwell 侵袭分析、特异性葡萄糖、乳酸或三磷酸腺苷测定试剂盒和 Western blot 检测细胞增殖、侵袭和糖酵解。通过双荧光素酶报告分析分析 miR-432-5p 与 circ-PTN 或 RAB10 的关系。通过小鼠异种移植模型探索 circ-PTN 对体内神经胶质瘤发展的影响。
circ-PTN 在神经胶质瘤组织和细胞中表达增强，miR-432-5p 丰度降低。circ-PTN 沉默抑制细胞增殖、侵袭和糖酵解。circ-PTN 通过直接海绵吸附 miR-432-5p 调节神经胶质瘤的发展。RAB10 是 miR-432-5p 的靶点，miR-432-5p 通过靶向 RAB10 抑制细胞增殖、侵袭和糖酵解。circ-PTN 可以通过 miR-432-5p 调节 RAB10 的表达。circ-PTN 敲低减少了体内神经胶质瘤细胞异种移植瘤的生长。
circ-PTN 敲低通过调节 miR-432-5p 和 RAB10 抑制神经胶质瘤中的细胞增殖、侵袭和糖酵解。
