Yi Xianrong, Zhao Biying, Tang Yan, Xu Zhimei
Guangxi Academy of Specialty Crops, Guilin 541004, China.
Guangxi Academy of Specialty Crops, Guilin 541004, China.
Genomics. 2020 Nov;112(6):3933-3942. doi: 10.1016/j.ygeno.2020.06.048. Epub 2020 Jul 3.
Pear is a traditional and economically fruit tree worldwide. With the development of the pear industry, pear fruit post-harvest preservation techniques have become very important. Among them, low temperature preservation technology is most widely used, but the molecular mechanism underlying this process is still unclear. To better understand this, RNA-seq was performed on samples collected at different time points with increasing storage time. Here, 19,610 differentially expressed genes were obtained and annotated into 51 GO terms and 26 KEGG-defined significantly overrepresented pathways. 2475 transcription factors belonging to 50 different families were identified with increasing storage time. Ethylene content increased with storage time and was the highest at 105 days of fruit storage. Accordingly, integrative analysis of gene expression revealed that 14 unigenes were related to the ethylene metabolic pathway. This study provides valuable resources to investigate the genetics of the ethylene metabolic pathways and improve pear storage and preservation technology.
梨是全球一种传统且具有经济价值的果树。随着梨产业的发展，梨果实采后保鲜技术变得非常重要。其中，低温保鲜技术应用最为广泛，但这一过程背后的分子机制仍不清楚。为了更好地理解这一点，对随着贮藏时间增加在不同时间点采集的样本进行了RNA测序。在此，获得了19610个差异表达基因，并注释到51个基因本体（GO）术语和26个KEGG定义的显著富集途径中。随着贮藏时间增加，鉴定出属于50个不同家族的2475个转录因子。乙烯含量随贮藏时间增加而增加，在果实贮藏105天时最高。因此，基因表达的综合分析表明，14个单基因与乙烯代谢途径相关。本研究为研究乙烯代谢途径的遗传学以及改进梨的贮藏和保鲜技术提供了有价值的资源。
