Palomino-Hermosillo Yolotzin Apatzingan, Berumen-Varela Guillermo, Ochoa-Jiménez Verónica Alhelí, Balois-Morales Rosendo, Jiménez-Zurita José Orlando, Bautista-Rosales Pedro Ulises, Martínez-González Mónica Elizabeth, López-Guzmán Graciela Guadalupe, Cortés-Cruz Moisés Alberto, Guzmán Luis Felipe, Cornejo-Granados Fernanda, Gallardo-Becerra Luigui, Ochoa-Leyva Adrian, Alia-Tejacal Iran
Unidad de Tecnología de Alimentos-Secretaría de Investigación y Posgrado, Universidad Autónoma de Nayarit, Ciudad de la Cultura SN, Tepic 63000, Nayarit, Mexico.
Unidad Académica de Agricultura, Universidad Autónoma de Nayarit, Carretera Tepic-Compostela, Km. 9, Xalisco 63780, Nayarit, Mexico.
Plants (Basel). 2022 Jul 7;11(14):1798. doi: 10.3390/plants11141798.
Soursop ( L.) is climacteric fruit with a short ripening period and postharvest shelf life, leading to a rapid softening. In this study, transcriptome analysis of soursop fruits was performed to identify key gene families involved in ripening under postharvest storage conditions (Day 0, Day 3 stored at 28 ± 2 °C, Day 6 at 28 ± 2 °C, Day 3 at 15 ± 2 °C, Day 6 at 15 ± 2 °C, Day 9 at 15 ± 2 °C). The transcriptome analysis showed 224,074 transcripts assembled clustering into 95, 832 unigenes, of which 21, 494 had ORF. RNA-seq analysis showed the highest number of differentially expressed genes on Day 9 at 15 ± 2 °C with 9291 genes (4772 up-regulated and 4519 down-regulated), recording the highest logarithmic fold change in pectin-related genes. Enrichment analysis presented significantly represented GO terms and KEGG pathways associated with molecular function, metabolic process, catalytic activity, biological process terms, as well as biosynthesis of secondary metabolites, plant hormone signal, starch, and sucrose metabolism, plant-pathogen interaction, plant-hormone signal transduction, and MAPK-signaling pathways, among others. Network analysis revealed that pectinesterase genes directly regulate the loss of firmness in fruits stored at 15 ± 2 °C.
刺果番荔枝（Annona muricata L.）是一种呼吸跃变型果实，成熟周期短，采后货架期短，导致果实迅速软化。在本研究中，对刺果番荔枝果实进行了转录组分析，以确定在采后储存条件下（第0天、28±2℃储存3天、28±2℃储存6天、15±2℃储存3天、15±2℃储存6天、15±2℃储存9天）参与成熟过程的关键基因家族。转录组分析显示，共组装了224,074条转录本，聚类为95,832个单基因，其中21,494个具有开放阅读框（ORF）。RNA测序分析表明，在15±2℃储存9天时差异表达基因数量最多，有9291个基因（4772个上调，4519个下调），果胶相关基因的对数倍变化最高。富集分析显示，显著代表的基因本体（GO）术语和京都基因与基因组百科全书（KEGG）通路与分子功能、代谢过程、催化活性、生物学过程术语以及次生代谢物生物合成、植物激素信号、淀粉和蔗糖代谢、植物-病原体相互作用、植物激素信号转导和丝裂原活化蛋白激酶（MAPK）信号通路等有关。网络分析表明，果胶酯酶基因直接调控15±2℃储存果实的硬度损失。
