Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil; Programa de Pós-Graduação em Ciências Fisiológicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil.
Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil.
J Photochem Photobiol B. 2020 Aug;209:111941. doi: 10.1016/j.jphotobiol.2020.111941. Epub 2020 Jun 28.
The visual system is an important biological indicator of effects induced by ultraviolet (UV) radiation. However, research has extensively investigated the effects of high-dose UV radiation in a single exposure, thus, the differential of this work was to investigate the effects of UVB radiation in low doses in single and repeated exposure. Therefore, we investigated the effects of repeated exposure to environmental UVB doses (0.09 J/cm) on the retina and optic lobes of the crab Neohelice granulata. We evaluated the reactive oxygen species (ROS) concentration, antioxidant capacity against peroxyl radicals (ACAP) levels, catalase (CAT) and glutathione S-transferase (GST) activities and lipoperoxidation (LPO) levels and performed histological analysis. The crabs were exposed to UVB radiation for 1 or 60 days, while the control group was exposed to visible light. In the retina region, increases in ROS concentration and CAT and GST activities after the single exposure were observed. After 60 days of exposure, we observed an increase in ACAP levels. In the optic lobes, we observed an increase in GST activity and a decrease in LPO levels after the single exposure. However, we observed an increase in ROS concentration after 60 days of exposure. Moreover, after 60 days of exposure, infiltrating hemocytes in the retina and disorganization in neuron cell bodies of the external medulla were observed. In this sense, single and repeated exposure to low doses of UVB radiation induced changes in oxidative status and inflammatory process in the visual system of the crab Neohelice granulata.
视觉系统是紫外线 (UV) 辐射诱导效应的一个重要生物指标。然而，研究已经广泛研究了单次高剂量 UV 辐射的影响，因此，这项工作的不同之处在于研究低剂量 UVB 辐射在单次和重复暴露下的影响。因此，我们研究了重复暴露于环境 UVB 剂量（0.09 J/cm）对蟹 Neohelice granulata 的视网膜和视神经叶的影响。我们评估了活性氧 (ROS) 浓度、抗过氧自由基能力 (ACAP) 水平、过氧化氢酶 (CAT) 和谷胱甘肽 S-转移酶 (GST) 活性和脂质过氧化 (LPO) 水平，并进行了组织学分析。螃蟹暴露于 UVB 辐射 1 或 60 天，而对照组暴露于可见光。在视网膜区域，单次暴露后 ROS 浓度和 CAT 和 GST 活性增加。暴露 60 天后，我们观察到 ACAP 水平增加。在视神经叶中，我们观察到单次暴露后 GST 活性增加和 LPO 水平降低。然而，暴露 60 天后，ROS 浓度增加。此外，暴露 60 天后，在视网膜中观察到浸润性血细胞和外髓质神经元细胞体的紊乱。从这个意义上说，单次和重复暴露于低剂量 UVB 辐射会导致蟹 Neohelice granulata 的视觉系统氧化状态和炎症过程发生变化。
