Kever L V, Komissarchik Ia Iu, Korolev E V
Tsitologiia. 1988 May;30(5):524-31.
The polyene antibiotic filipin has been used to characterize the cholesterol distribution in the membranes of resting and ADH-stimulated frog urinary bladder in freeze-fracture replicas. In general, the intracellular membranes takes up filipin only insignificantly. An exception is the cholesterol rich granule membrane. Both density and polarity of filipin-induced deformations were evaluated, and the asymmetry in membrane cholesterol was analysed. Upon ADH-stimulation of water flow both density and polarity of filipin-induced deformations altered differently in apical and basolateral regions of the plasma membrane. This difference is presumably due to the stretching of the basolateral membrane as a result of swelling, on the one hand, and to incorporation of aggregate containing membranes into the apical membrane, on the other one. The results obtained may suggest that the appearance of ADH-induced intramembranous particle aggregates in the apical membrane be accompanied with a relative cholesterol decrease in this apical membrane.
多烯抗生素制霉菌素已被用于在冷冻蚀刻复制品中表征静息和抗利尿激素刺激的青蛙膀胱膜中的胆固醇分布。一般来说，细胞内膜对制霉菌素的摄取微不足道。富含胆固醇的颗粒膜是个例外。评估了制霉菌素诱导变形的密度和极性，并分析了膜胆固醇的不对称性。抗利尿激素刺激水流后，质膜顶端和基底外侧区域制霉菌素诱导变形的密度和极性发生了不同变化。这种差异可能一方面是由于肿胀导致基底外侧膜伸展，另一方面是由于含聚集物的膜融入顶端膜。所得结果可能表明，顶端膜中抗利尿激素诱导的膜内颗粒聚集物出现时，该顶端膜中的胆固醇相对减少。
