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[蛙膀胱上皮细胞顶端细胞骨架的超微结构研究:抗利尿激素依赖性和非依赖性渗透通透性增加]

[An ultrastructural study of the apical cytoskeleton of the epithelial cells in the frog bladder with an ADH-dependent and an ADH-independent increase in osmotic permeability].

作者信息

Komissarchik Ia Iu, Makarenkova E I, Snigirevskaia E S, Shakhmatova E I, Brudnaia M S, Natochin Iu V

出版信息

Tsitologiia. 1996;38(9):927-33.

PMID:9019895
Abstract

Immunocytochemical methods of electron and confocal microscopy were applied for studying the primembrane actin cytoskeleton in the frog urinary bladder granular cells, following the two actions: under the increased vasopressin-induced water permeability, and following autacoid removal by multiple changes of the Ringer solution around the serosa. In both cases similar changes have been revealed in the structure of the apical cytoskeleton and, in addition, a decrease in the density of its actin filament distribution was noticed.

摘要

运用电子显微镜和共聚焦显微镜的免疫细胞化学方法,对蛙膀胱颗粒细胞的原膜肌动蛋白细胞骨架进行研究,采取了以下两种操作:一是在加压素诱导水通透性增加的情况下,二是在通过多次更换浆膜周围的林格溶液去除自分泌物质之后。在这两种情况下,顶端细胞骨架的结构都出现了类似变化,此外,还注意到其肌动蛋白丝分布密度降低。

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