Heuer H, Casals-Stenzel J
Department of Pharmacology, Boehringer Ingelheim KG, Federal Republic of Germany.
Agents Actions Suppl. 1988;23:207-15. doi: 10.1007/978-3-0348-9156-1_16.
The specific and potent antagonist of platelet activating factor (PAF), WEB 2086, has been used to investigate the putative role of PAF in anaphylaxis in the guinea-pig. Intravenous (i.v.) challenge with ovalbumin has been compared to inhalative provocation. When actively sensitized guinea-pigs were challenged by the i.v. route, in the presence of mepyramine (5 micrograms/kg i.v.), oral WEB 2086 (0.05-0.5 mg/kg) inhibited the anaphylactic bronchoconstriction but not the hypotension. In passively sensitized guinea-pigs, i.v. WEB 2086, in the presence of mepyramine, protected the animals from both anaphylactic bronchoconstriction and blood pressure changes. When the antigen was inhaled by actively sensitized guinea-pigs, WEB 2086 inhibited the anaphylactic reaction without the administration of any additional antihistamine. These results suggest that PAF plays an important role in anaphylaxis. PAF may be (a) more important in passive than in active anaphylaxis and (b) may be involved during inhalative challenge in active anaphylaxis.
血小板活化因子（PAF）的特异性强效拮抗剂WEB 2086已被用于研究PAF在豚鼠过敏反应中的假定作用。已将卵清蛋白静脉内（i.v.）激发与吸入性激发进行了比较。当对主动致敏的豚鼠进行静脉途径激发时，在存在甲氧苄胺（5微克/千克静脉内）的情况下，口服WEB 2086（0.05 - 0.5毫克/千克）可抑制过敏反应性支气管收缩，但不能抑制低血压。在被动致敏的豚鼠中，在存在甲氧苄胺的情况下，静脉内给予WEB 2086可保护动物免受过敏反应性支气管收缩和血压变化的影响。当主动致敏的豚鼠吸入抗原时，WEB 2086在不给予任何额外抗组胺药的情况下即可抑制过敏反应。这些结果表明，PAF在过敏反应中起重要作用。PAF可能（a）在被动过敏反应中比在主动过敏反应中更重要，并且（b）可能参与主动过敏反应中的吸入性激发过程。
