细胞固定：两个简单技巧。

Koonce Michael, Tikhonenko Irina, Gräf Ralph

Division of Translational Medicine, Wadsworth Center, NYS Department of Health, Albany, NY 12237, USA.

Department of Cell Biology, University of Potsdam, Potsdam-Golm 14476, Germany.

Methods Protoc. 2020 Jul 1;3(3):47. doi: 10.3390/mps3030047.

We share two simple modifications to enhance the fixation and imaging of relatively small, motile, and rounded model cells. These include cell centrifugation and the addition of trace amounts of glutaraldehyde to existing fixation methods. Though they need to be carefully considered in each context, they have been useful to our studies of the spatial relationships of the microtubule cytoskeletal system.

我们分享两种简单的改良方法，以增强相对较小、可移动且呈圆形的模型细胞的固定和成像效果。这些方法包括细胞离心以及在现有固定方法中添加微量戊二醛。尽管在每种情况下都需要仔细考虑，但它们对我们研究微管细胞骨架系统的空间关系很有帮助。