Institute for Cell and Molecular Biosciences, University of Newcastle, Newcastle upon Tyne, NE2 4HH, UK.
FG11 Division of Enteropathogenic Bacteria and Legionella, Robert Koch Institute, Burgstrasse 37, 38855, Wernigerode, Germany.
Nat Commun. 2019 Jan 16;10(1):261. doi: 10.1038/s41467-018-08056-2.
Bacterial growth and cell division requires precise spatiotemporal regulation of the synthesis and remodelling of the peptidoglycan layer that surrounds the cytoplasmic membrane. GpsB is a cytosolic protein that affects cell wall synthesis by binding cytoplasmic mini-domains of peptidoglycan synthases to ensure their correct subcellular localisation. Here, we describe critical structural features for the interaction of GpsB with peptidoglycan synthases from three bacterial species (Bacillus subtilis, Listeria monocytogenes and Streptococcus pneumoniae) and suggest their importance for cell wall growth and viability in L. monocytogenes and S. pneumoniae. We use these structural motifs to identify novel partners of GpsB in B. subtilis and extend the members of the GpsB interactome in all three bacterial species. Our results support that GpsB functions as an adaptor protein that mediates the interaction between membrane proteins, scaffolding proteins, signalling proteins and enzymes to generate larger protein complexes at specific sites in a bacterial cell cycle-dependent manner.
细菌的生长和细胞分裂需要精确的时空调节，以合成和重塑围绕细胞质膜的肽聚糖层。GpsB 是一种细胞质蛋白，通过与肽聚糖合成酶的细胞质小域结合，确保其正确的亚细胞定位，从而影响细胞壁的合成。在这里，我们描述了 GpsB 与来自三种细菌（枯草芽孢杆菌、单核细胞增生李斯特菌和肺炎链球菌）的肽聚糖合成酶相互作用的关键结构特征，并提出了它们对单核细胞增生李斯特菌和肺炎链球菌细胞壁生长和活力的重要性。我们利用这些结构基序在枯草芽孢杆菌中鉴定了 GpsB 的新伴侣，并扩展了所有三种细菌中 GpsB 相互作用组的成员。我们的结果支持 GpsB 作为一种衔接蛋白的功能，它介导膜蛋白、支架蛋白、信号蛋白和酶之间的相互作用，以在细菌细胞周期依赖性的方式在特定部位生成更大的蛋白质复合物。
