Miyauchi M, Shionoya S
First Department of Surgery, Nagoya University School of Medicine, Japan.
Angiology. 1988 Oct;39(10):881-90. doi: 10.1177/000331978803901005.
This study, using C3a, C4a, and C5a assay and crossed immunoelectrophoresis in vitro and in vivo, was designed to determine whether vascular prostheses activate the complement system. The degree of complement activation and the pathway of activation were also studied. The complement levels (C3, C4, CH50) in different arterial diseases were measured, and the relationship of complement levels to serum lipids in patients with arteriosclerosis obliterans (ASO) was also investigated, in order to clarify a relationship between the complement system and arteriosclerosis or other arterial diseases. Bovine graft, dacron, and PTFE activated the complement system in vitro. In order of the degree of complement activation, bovine graft ranked first, followed by dacron and PTFE. The patient group with acute arterial occlusion showed high C3 and C4 levels. The healthy elderly group showed higher C3 and C4 levels than the healthy persons in their twenties. In ASO patients, C3 levels correlated with serum lipid levels. Judged from the relation of complement activation by vascular prostheses to the progression of arteriosclerotic changes, vascular prostheses should be assessed with regard to complement activation.
本研究采用C3a、C4a和C5a检测以及体内外交叉免疫电泳法，旨在确定血管假体是否激活补体系统。同时还研究了补体激活的程度和激活途径。测量了不同动脉疾病中的补体水平（C3、C4、CH50），并调查了闭塞性动脉硬化症（ASO）患者补体水平与血脂的关系，以阐明补体系统与动脉硬化或其他动脉疾病之间的关系。牛移植血管、涤纶和聚四氟乙烯在体外激活补体系统。按补体激活程度排序，牛移植血管居首，其次是涤纶和聚四氟乙烯。急性动脉闭塞患者组的C3和C4水平较高。健康老年组的C3和C4水平高于20多岁的健康人群。在ASO患者中，C3水平与血脂水平相关。从血管假体激活补体与动脉硬化变化进展的关系判断，应评估血管假体的补体激活情况。
