Department of Food Science and Technology, Seoul National University of Science & Technology, Seoul 01811, Korea.
Department of Food Science and Nutrition, KC University, Seoul 07661, Korea.
Nutrients. 2020 Jul 2;12(7):1967. doi: 10.3390/nu12071967.
Yeonsan Ogye is a traditional Korean chicken breed , GD), with a dominant gene for fibromelanosis, showing entirely black fluffy head feathers, ear lobes, and pupils. GD collagen extract (78.6 g per 100 g total protein) was derived from the flesh of Yeonsan Ogye. The effects of GD collagen on bone mass, microarchitecture, osteogenic, osteoclastogenic differentiations, and function factor expression were investigated in ovariectomized (OVX) rats. GD collagen stimulated osteogenesis in OVX rats and increased tibial bone strength and calcium content. Micro-computed tomography analysis of tibia cross-sections revealed that GD collagen attenuated the OVX-induced changes in trabecular thickness, spacing, and number. GD collagen stimulated alkaline phosphatase activity, bone-specific matrix proteins (alkaline phosphatase (ALP), osteocalcin, collagen type I (COL-I)) and mineralization by activating bone morphogenetic protein 2 (BMP-2)/mothers against decapentaplegic homolog 5 (SMAD5)/runt-related transcription factor 2 (Runx2). GD collagen inhibited osteoclast differentiation and function gene markers (TRAP, cathepsin K) by interfering with the Wnt signaling, increasing OPG production, and reducing the expression of RANKL, TRAP, and cathepsin K. GD collagen promoted osteogenesis by activating the p38 signal pathway and prevented osteoclastogenesis by lowering the RANKL/OPG ratio and blocking the JNK signaling pathway. Dietary supplementation with GD collagen might inhibit osteoclastogenesis, stimulate osteoblastogenesis, and regulate bone metabolism.
延山乌鸡是一种具有纤维黑色素沉着显性基因的韩国传统鸡种，其头部羽毛、耳垂和瞳孔均为黑色绒毛状。GD 胶原蛋白提取物（每 100g 总蛋白中含有 78.6g）源自延山乌鸡的肉。本研究旨在探讨 GD 胶原蛋白对去卵巢（OVX）大鼠骨量、微结构、成骨细胞、破骨细胞分化及功能因子表达的影响。GD 胶原蛋白可刺激 OVX 大鼠的成骨作用，增加胫骨骨强度和钙含量。胫骨横断面的微计算机断层扫描分析表明，GD 胶原蛋白可减轻 OVX 引起的骨小梁厚度、间距和数量的变化。GD 胶原蛋白通过激活骨形态发生蛋白 2（BMP-2）/母亲对抗 decapentaplegic 同源物 5（SMAD5）/ Runt 相关转录因子 2（Runx2），刺激碱性磷酸酶活性、骨特异性基质蛋白（碱性磷酸酶（ALP）、骨钙素、I 型胶原（COL-I））和矿化。GD 胶原蛋白通过干扰 Wnt 信号通路、增加 OPG 产生和降低 RANKL、TRAP 和组织蛋白酶 K 的表达，抑制破骨细胞分化和功能基因标志物（TRAP、组织蛋白酶 K）。GD 胶原蛋白通过激活 p38 信号通路促进成骨作用，通过降低 RANKL/OPG 比值和阻断 JNK 信号通路来防止破骨细胞生成。膳食补充 GD 胶原蛋白可能抑制破骨细胞生成、刺激成骨细胞生成，并调节骨代谢。
