Yoo Han Seok, Kim Gyung-Ji, Song Da Hye, Chung Kang-Hyun, Lee Kwon-Jai, Kim Dong-Hee, An Jeung Hee
Department of Food Science and Technology, Seoul National University of Science & Technology, Seoul 01811, Korea.
Department of Chemical & Biomolecular Engineering, Sogang University, Seoul 04170, Korea.
Nutrients. 2017 May 17;9(5):504. doi: 10.3390/nu9050504.
The present study evaluated the effects of a calcium (Ca) supplement derived from (GD) on breaking force, microarchitecture, osteogenic differentiation and osteoclast differentiation factor expression in vivo in Ca-deficient ovariectomized (OVX) rats. One percent of Ca supplement significantly improved Ca content and bone strength of the tibia. In micro-computed tomography analysis, 1% Ca supplement attenuated OVX- and low Ca-associated changes in bone mineral density, trabecular thickness, spacing and number. Moreover, 1% Ca-supplemented diet increased the expression of osteoblast differentiation marker genes, such as bone morphogenetic protein-2, Wnt3a, small mothers against decapentaplegic 1/5/8, runt-related transcription factor 2, osteocalcin and collagenase-1, while it decreased the expression of osteoclast differentiation genes, such as thrombospondin-related anonymous protein, cathepsin K and receptor activator of nuclear factor kappa B. Furthermore, 1% Ca-supplemented diet increased the levels of phosphorylated extracellular signal-regulated kinase and c-Jun N-terminal kinase. The increased expression of osteoblast differentiation marker genes and activation of mitogen-activated protein kinase signaling were associated with significant increases in trabecular bone volume, which plays an important role in the overall skeletal strength. Our results demonstrated that 1% Ca supplement inhibited osteoclastogenesis, stimulated osteoblastogenesis and restored bone loss in OVX rats.
本研究评估了源自[具体来源未给出]（GD）的钙补充剂对缺钙去卵巢（OVX）大鼠体内骨折力、微观结构、成骨分化和破骨细胞分化因子表达的影响。1%的钙补充剂显著提高了胫骨的钙含量和骨强度。在微型计算机断层扫描分析中，1%的钙补充剂减轻了OVX和低钙相关的骨矿物质密度、小梁厚度、间距和数量的变化。此外，1%钙补充饮食增加了成骨细胞分化标记基因的表达，如骨形态发生蛋白-2、Wnt3a、小母亲抗五肢瘫痪蛋白1/5/8、 runt相关转录因子2、骨钙素和胶原酶-1，同时降低了破骨细胞分化基因的表达，如血小板反应蛋白相关无名蛋白、组织蛋白酶K和核因子κB受体激活剂。此外，1%钙补充饮食增加了磷酸化细胞外信号调节激酶和c-Jun N末端激酶的水平。成骨细胞分化标记基因表达的增加和丝裂原活化蛋白激酶信号的激活与小梁骨体积的显著增加相关，小梁骨体积在整体骨骼强度中起重要作用。我们的结果表明，1%的钙补充剂抑制了OVX大鼠的破骨细胞生成，刺激了成骨细胞生成并恢复了骨质流失。
