College of Pharmacy, Gachon University, Incheon 21936, Korea.
Basilbiotech, Seoul 06621, Korea.
Molecules. 2020 Jul 2;25(13):3028. doi: 10.3390/molecules25133028.
is one of the most abundant marine planktons, and it has a crucial feature in the carbon cycle. However, proteomic analyses of have not been done extensively. In this study, a three-dimensional liquid chromatography (3D-LC) system consisting of strong cation exchange, high- and low-pH reversed-phase liquid chromatography was established for in-depth proteomic profiling of . From tryptic proteome digest, 70 fractions were generated and analyzed using liquid chromatography-tandem mass spectrometry. In total, more than 84,000 unique peptides and 10,000 proteins groups were identified with a false discovery rate of ≤0.01. The physicochemical properties of the identified peptides were evaluated. Using ClueGO, approximately 700 gene ontology terms and 15 pathways were defined from the identified protein groups with -value ≤0.05, covering a wide range of biological processes, cellular components, and molecular functions. Many biological processes associated with CO fixation, photosynthesis, biosynthesis, and metabolic process were identified. Various molecular functions relating to protein binding and enzyme activities were also found. The 3D-LC strategy is a powerful approach for comparative proteomic studies on to reveal changes in its protein level and related mechanism.
是最丰富的海洋浮游生物之一，在碳循环中具有关键特征。然而，对 的蛋白质组学分析尚未广泛开展。在这项研究中，建立了一个由强阳离子交换、高和低 pH 值反相液相色谱组成的三维液相色谱 (3D-LC) 系统，用于 的深入蛋白质组学分析。从胰蛋白酶酶切产物中，生成了 70 个馏分，并使用液相色谱-串联质谱进行分析。总共鉴定出超过 84000 个独特肽段和 10000 个蛋白质组，假发现率≤0.01。评估了鉴定出的肽段的物理化学性质。使用 ClueGO，从鉴定的蛋白质组中定义了大约 700 个基因本体术语和 15 条途径，-值≤0.05，涵盖了广泛的生物学过程、细胞成分和分子功能。鉴定出许多与 CO 固定、光合作用、生物合成和代谢过程相关的生物学过程。还发现了与蛋白质结合和酶活性相关的各种分子功能。3D-LC 策略是研究 蛋白质组变化及其相关机制的强大方法。
