Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Science, Novosibirsk, Russia.
Omsk Research Institute of Natural Focal Infections, Omsk, Russia.
Ticks Tick Borne Dis. 2020 Sep;11(5):101499. doi: 10.1016/j.ttbdis.2020.101499. Epub 2020 Jun 28.
A total of 705 rodents from Myodes, Microtus, and Apodemus genera, 396 adult questing Ixodes persulcatus, and 115 Ixodes larvae and nymphs taken from rodents (and then molted under laboratory conditions to nymphs and adults) were collected in 2013-2018 in Omsk province, Russian Siberia, and examined for the presence of Anaplasmataceae. DNA of Anaplasma phagocytophilum was detected in 29.5 % rodents, 3.8 % questing I. persulcatus, two molted adult I. persulcatus, and one molted adult Ixodes trianguliceps. Ehrlichia muris DNA was found in specimens from 12.1 % rodents, 3.0 % questing I. persulcatus, 14 % molted adult I. persulcatus, and one molted adult I. trianguliceps. Neoehrlichia mikurensis DNA was found in 0.6 % blood samples. It was suggested that in the studied area A. phagocytophilum and E. muris are mainly transmitted to small rodents by I. trianguliceps and I. persulcatus, respectively. Based on groEL gene sequence analysis, three phylogenetic clusters of A. phagocytophilum (clusters 4, 5, 6, according to Jaarsma et al., 2019) were identified. Most of genotyped A. phagocytophilum isolates obtained from rodents (87.6 %) and a single isolate found in a molted adult I. trianguliceps belonged to cluster 5. Cluster 6 contained 11.8 % genotyped specimens from rodents, and one questing and two molted adult I. persulcatus, while cluster 4 included specimens from 93 % genotyped questing I. persulcatus and one vole. The finding of A. phagocytophilum from clusters 5 and 6 in voles from the same sampling area indicated that clusters 5 and 6 segregate according to the tick-carriers, but not the geography. Most of the genotyped specimens of E. muris and N. mikurensis corresponded to typical genotypes detected in Asian Russia previously. In addition, new genetic variants of E. muris and N. mikurensis, which significantly differed from other known isolates and formed separate branches on phylogenetic trees, were identified in several voles.
在 2013-2018 年，从俄罗斯西伯利亚的鄂木斯克省共采集了 705 只来自 Myodes、Microtus 和 Apodemus 属的啮齿动物、396 只成年硬蜱（然后在实验室条件下蜕皮成若虫和成虫）以及 115 只幼虫和若虫，以检测有无立克次体科。在 29.5%的啮齿动物、3.8%的硬蜱（待刺吸）、2 只蜕皮的成虫硬蜱和 1 只蜕皮的成虫三棘血蜱中检测到嗜吞噬细胞无形体 DNA。在 12.1%的啮齿动物、3.0%的硬蜱（待刺吸）、14%的蜕皮成虫硬蜱和 1 只蜕皮成虫三棘血蜱中发现了鼠埃立克体 DNA。在 0.6%的血液样本中发现了新壁衣支原体 DNA。研究表明，在研究区域内，嗜吞噬细胞无形体和鼠埃立克体主要通过三棘血蜱和硬蜱（待刺吸）传播给小型啮齿动物。根据 groEL 基因序列分析，鉴定出嗜吞噬细胞无形体的三个进化群（根据 Jaarsma 等人，2019 年为群 4、5、6）。从啮齿动物中获得的大多数基因分型嗜吞噬细胞无形体分离株（87.6%）和从蜕皮成虫三棘血蜱中发现的单个分离株属于群 5。群 6 包含 11.8%基因分型的来自啮齿动物的标本，以及 1 只硬蜱（待刺吸）和 2 只蜕皮成虫硬蜱，而群 4 包含 93%基因分型的硬蜱（待刺吸）和 1 只田鼠。在同一采样区域从田鼠中发现的群 5 和 6 的嗜吞噬细胞无形体表明，群 5 和 6 根据蜱虫载体而不是地理位置进行分离。大多数基因分型的鼠埃立克体和新壁衣支原体标本与以前在亚洲俄罗斯检测到的典型基因型相对应。此外，在几只田鼠中还鉴定到了新的鼠埃立克体和新壁衣支原体的遗传变异体，它们与其他已知分离株明显不同，并在系统发育树上形成了单独的分支。
