Chang Weipang, Pedroni Andrea, Hohendorf Victoria, Giacomello Stefania, Hibi Masahiko, Köster Reinhard W, Ampatzis Konstantinos
Department of Neuroscience, Karolinska Institutet, Stockholm 17177, Sweden.
SciLifeLab, Royal Institute of Technology (KTH), Stockholm 17165, Sweden.
Proc Natl Acad Sci U S A. 2020 Jul 21;117(29):17330-17337. doi: 10.1073/pnas.2005633117. Epub 2020 Jul 6.
Purkinje cells, the principal neurons of cerebellar computations, are believed to comprise a uniform neuronal population of cells, each with similar functional properties. Here, we show an undiscovered heterogeneity of adult zebrafish Purkinje cells, revealing the existence of anatomically and functionally distinct cell types. Dual patch-clamp recordings showed that the cerebellar circuit contains all Purkinje cell types that cross-communicate extensively using chemical and electrical synapses. Further activation of spinal central pattern generators (CPGs) revealed unique phase-locked activity from each Purkinje cell type during the locomotor cycle. Thus, we show intricately organized Purkinje cell networks in the adult zebrafish cerebellum that encode the locomotion rhythm differentially, and we suggest that these organizational properties may also apply to other cerebellar functions.
浦肯野细胞是小脑计算的主要神经元，被认为由一群功能特性相似的统一神经元组成。在此，我们展示了成年斑马鱼浦肯野细胞未被发现的异质性，揭示了解剖学和功能上不同细胞类型的存在。双膜片钳记录表明，小脑回路包含所有类型的浦肯野细胞，它们通过化学和电突触进行广泛的交叉通信。脊髓中枢模式发生器（CPG）的进一步激活揭示了在运动周期中每种浦肯野细胞类型独特的锁相活动。因此，我们展示了成年斑马鱼小脑中错综复杂的浦肯野细胞网络，它们以不同方式编码运动节律，并且我们认为这些组织特性也可能适用于其他小脑功能。
