Leptospirosis Research and Expertise Unit, Institut Pasteur in New Caledonia, Institut Pasteur International Network, Noumea, New Caledonia.
Methods Mol Biol. 2020;2134:1-9. doi: 10.1007/978-1-0716-0459-5_1.
Leptospires, the etiological agents of leptospirosis, are fastidious slow-growing organisms. Here we describe the isolation and routine maintenance of leptospires from clinical (blood, urine, or tissue) and environmental (water or soil) samples. Using combinations of filtration, agar plating, and selective agents, leptospires can be isolated in pure cultures even from complex contaminated sources in standard EMJH culture medium.
钩端螺旋体，钩端螺旋体病的病原体，是一种苛刻的缓慢生长的生物体。在这里，我们描述了从临床（血液、尿液或组织）和环境（水或土壤）样本中分离和常规维持钩端螺旋体的方法。通过过滤、琼脂平板和选择性试剂的组合，可以在标准的 EMJH 培养基中从复杂的污染源中分离出纯培养的钩端螺旋体。
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