Zuerner Richard L
National Animal Disease Center USDA, ARS, MWA, Ames, Iowa, USA.
Curr Protoc Microbiol. 2005 Oct;Chapter 12:Unit 12E.1. doi: 10.1002/9780471729259.mc12e01s00.
Analysis of Leptospira requires use of specialized media for growth, maintenance, and storage of viable bacteria that can be used in experimental protocols in a predictable manner. However, pathogenic Leptospira are fastidious bacteria with unusual nutritional requirements. These problems make primary isolation, routine propagation, and storage of Leptospira difficult. Defined and complex media are available for routine growth of pathogenic Leptospira, and each medium has unique characteristics that favor the growth or isolation of some strains, while not supporting the growth of others. This unit will describe the preparation of Leptospira media and methods for isolation and storage of these bacteria.
钩端螺旋体的分析需要使用专门的培养基来培养、维持和储存有活力的细菌，以便能够以可预测的方式用于实验方案。然而，致病性钩端螺旋体是营养要求特殊的苛求菌。这些问题使得钩端螺旋体的初次分离、常规传代培养和储存变得困难。有确定成分的培养基和复杂培养基可用于致病性钩端螺旋体的常规培养，每种培养基都有独特的特性，有利于某些菌株的生长或分离，而不支持其他菌株的生长。本单元将描述钩端螺旋体培养基的制备以及这些细菌的分离和储存方法。
