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从标记为“用于组织培养的无菌”的市售去离子水中分离出双曲钩端螺旋体。

Isolation of Leptospira biflexa from commercially prepared deionized water labeled "sterile for tissue culture".

作者信息

Rubin S J, Perlman S, Ellinghausen H C

出版信息

J Clin Microbiol. 1980 Jul;12(1):121-3. doi: 10.1128/jcm.12.1.121-123.1980.

Abstract

Leptospira biflexa were isolated from urine cultures of two patients with clinical and laboratory findings compatible with leptospirosis. Neither patient had detectable leptospiral agglutinins. The source of the L. biflexa was water labeled " "sterile for tissue culture" purchased from M. A. Bioproducts, formerly Microbological Associates. M. A. Bioproducts water is sterilized by filtration through a 0.22-mum-pore size membrane filter. Leptospiral contamination can occur in products sterilized by filtration. Heat sterilization of water eliminates this possibility.

摘要

从两名临床和实验室检查结果符合钩端螺旋体病的患者尿液培养物中分离出双曲钩端螺旋体。两名患者均未检测到钩端螺旋体凝集素。双曲钩端螺旋体的来源是从M.A.生物制品公司(原微生物学协会)购买的标有“组织培养无菌”的水。M.A.生物制品公司的水通过0.22微米孔径的膜过滤器过滤灭菌。通过过滤灭菌的产品可能会发生钩端螺旋体污染。水的热灭菌消除了这种可能性。

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