Laboratorio de Desenvolvimento de Vacinas-Centro de Biotecnologia, Instituto Butantan, Sao Paulo, Brazil.
Methods Mol Biol. 2020;2134:171-185. doi: 10.1007/978-1-0716-0459-5_16.
The adhesion of pathogenic bacteria to host cells and the extracellular matrix (ECM) is considered an important step in the pathogenesis of microorganisms. It has been described that Leptospira spp. bind to multiple receptors on host cells and to the ECM to initiate infection. Most studies of Leptospira adherence described until now have focused on the in vitro attachment of recombinant L. interrogans proteins to ECM components. These putative adhesins may be involved in the colonization of the host, contributing to the bacterial invasion process. Certainly, in vitro cell adhesion studies have contributed to the elucidation of leptospiral pathogenesis mechanisms. Here, we describe a cell adhesion assay that can be used for studying the interactions between putative leptospiral adhesins and host components.
病原菌黏附于宿主细胞和细胞外基质（ECM）被认为是微生物发病机制中的重要步骤。已经描述了钩端螺旋体属（Leptospira spp.）通过与宿主细胞上的多种受体和 ECM 结合来启动感染。到目前为止，大多数关于钩端螺旋体黏附的研究都集中在重组 L. interrogans 蛋白与 ECM 成分的体外黏附上。这些假定的黏附素可能参与宿主的定植，有助于细菌的入侵过程。当然，体外细胞黏附研究有助于阐明钩端螺旋体的发病机制。在这里，我们描述了一种细胞黏附测定法，可用于研究假定的钩端螺旋体黏附素与宿主成分之间的相互作用。
