Department of Applied Biological Sciences, Jordan University of Science and Technology, Irbid, 22110, Jordan.
Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid, 22110, Jordan.
Pharmacol Rep. 2020 Dec;72(6):1717-1724. doi: 10.1007/s43440-020-00123-0. Epub 2020 Jul 6.
Synthetic cannabinoids (SCs) are a group of newly-developed drugs that bind and activate endocannabinoid system receptors. Angiogenesis is a biological process in which new blood vessels are formed from preexistent blood vessels. It plays a vital role in tissue growth, wound healing, and embryogenesis. This study aims to investigate the effects of the synthetic cannabinoid XLR-11 on specific cellular functions such as viability and angiogenesis in vitro.
Human brain microvascular endothelial cells (HBMECs) were cultured in DMEM/F12 medium supplemented with an endothelial cell growth kit. The MTT assay was used to investigate the viability of endothelial cells. An endothelial cell migration assay was used to investigate migration ability, while a tube formation assay was used to investigate the angiogenic capacity of the endothelial cells.
XLR-11 was found to enhance the viability of HBMECs. Moreover, the migration rate and angiogenic capacity significantly increased in the presence of various concentrations of XLR-11 compared to the control.
The current study shows that XLR-11 increases the viability of human brain microvascular endothelial cells and enhances angiogenesis in the brain in vitro, suggesting that XLR-11 could potentially be used as a therapeutic angiogenic drug in human brain injury treatment.
合成大麻素（SCs）是一组新开发的药物，可与内源性大麻素系统受体结合并激活它们。血管生成是一种新的血管从预先存在的血管形成的生物学过程。它在组织生长、伤口愈合和胚胎发生中起着至关重要的作用。本研究旨在探讨合成大麻素 XLR-11 对体外特定细胞功能（如活力和血管生成）的影响。
用人脑微血管内皮细胞（HBMEC）在含有内皮细胞生长试剂盒的 DMEM/F12 培养基中培养。使用 MTT 测定法来研究内皮细胞的活力。使用内皮细胞迁移测定法来研究迁移能力，而使用管形成测定法来研究内皮细胞的血管生成能力。
发现 XLR-11 增强了 HBMEC 的活力。此外，与对照组相比，存在各种浓度的 XLR-11 时，迁移率和血管生成能力显著增加。
目前的研究表明，XLR-11 增加了人脑微血管内皮细胞的活力并增强了体外脑内的血管生成，提示 XLR-11 可能可作为人类脑损伤治疗中的治疗性血管生成药物。
