Medical University of Lodz, Department of Biochemistry, Faculty of Medicine, Lodz, Poland.
Lodz University of Technology, Institute of Molecular and Industrial Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz, Poland.
J Physiol Pharmacol. 2020 Apr;71(2). doi: 10.26402/jpp.2020.2.05. Epub 2020 Jul 2.
Phenolic compounds are very important in the prevention and treatment of many civilization diseases, including colorectal cancer (CRC). In the present study we investigated and compared the phytochemical composition, antioxidant and cytotoxic activity of Japanese quince (Chaenomeles japonica L.) leaves crude phenolic extract (CPE) and purified phenolic-rich extracts (PRE). The UPLC-Q-TOF-MS analysis showed that both extracts contain diversified phenolics compounds (33 - 36 compounds in the PRE and CPE, respectively), among which chlorogenic acid and naringenin hexoside turned out to be the main constituents. Both FRAP and ABTS tests showed that PRE had 2-fold higher antioxidant activity compared to CPE. Furthermore, PRE exhibited a higher cytotoxic activity towards colon cancer cells (SW-480 and HT-29) than CPE. After 24-hours incubation with PRE the IC value for SW-480 cell line was obtained at the concentration of 239 μg/mL, while CPE treatment caused the same decrease only after 72h at 277 μg/mL. In addition, PRE had a stronger cytotoxic effect on the colon cancer cell lines (SW-480 and HT-29) than on normal intestinal cells (CCD-18Co and CCD 841 CoN). These results provide the first evidence that extracts from Japanese quince leaves (especially phenolic-rich extract, PRE) strongly decrease the viability of both SW-480 and HT-29 lines, which may suggest their cytotoxic activity towards colon cancer cells.
酚类化合物在预防和治疗多种文明病方面非常重要，包括结直肠癌（CRC）。在本研究中，我们研究并比较了皱皮木瓜（Chaenomeles japonica L.）叶粗酚提取物（CPE）和纯化的富含酚类提取物（PRE）的植物化学成分、抗氧化和细胞毒性活性。UPLC-Q-TOF-MS 分析表明，两种提取物均含有多样化的酚类化合物（PRE 和 CPE 中分别含有 33-36 种化合物），其中绿原酸和柚皮苷是主要成分。FRAP 和 ABTS 测试均表明，PRE 的抗氧化活性比 CPE 高 2 倍。此外，PRE 对结肠癌细胞（SW-480 和 HT-29）的细胞毒性活性高于 CPE。用 PRE 孵育 24 小时后，SW-480 细胞系的 IC 值为 239μg/mL，而 CPE 处理仅在 72 小时时浓度为 277μg/mL 时才会引起相同的降低。此外，PRE 对结肠癌细胞系（SW-480 和 HT-29）的细胞毒性作用强于对正常肠细胞（CCD-18Co 和 CCD 841 CoN）的作用。这些结果首次证明，皱皮木瓜叶提取物（特别是富含酚类的提取物 PRE）强烈降低了 SW-480 和 HT-29 两种细胞系的活力，这可能表明它们对结肠癌细胞的细胞毒性活性。
