Medical University of Lodz, Department of Biochemistry, Faculty of Medicine, Lodz, Poland.
Lodz University of Technology Institute of Molecular and Industrial Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz, Poland.
J Physiol Pharmacol. 2022 Apr;73(2). doi: 10.26402/jpp.2022.2.06. Epub 2022 Aug 18.
The objective of this study was to investigate the phytochemical composition, antioxidant and cytotoxic potential of aronia leaf crude phenolic-extract (ACE) and purified phenolic-rich extract (APE) on human intestinal cells (CCD 841 CoN) and colon cancer cells (SW-480 and HT-29). UPLC-Q-TOF-MS analysis confirmed that aronia leaves are rich in structurally diverse polyphenols (25 and 42 compounds for ACE and APE, respectively). Chlorogenic acid and quercetin-3-rutinoside were most abundant in both aronia extracts. The sum of detected polyphenols varied significantly between extracts ranging from 32.8 mg/g (ACE) to 436.3 mg/g (APE). The biological potential of aronia extracts was confirmed by applying in vitro antioxidant and cytotoxic assays. The results of antioxidant activity (ABTS and FRAP) indicate that APE showed 2-fold stronger antioxidant properties compared to ACE. APE revealed a stronger cytotoxic effect on SW-480 and HT-29 cells than ACE (MTT test). After 48 -hours of incubation, APE was found to inhibit SW-480 cell growth by 50% vs. control at 194.35 μg/mL, while for HT-29 cells it was observed at 552.02 μg/mL. In the case of ACE, IC has not been reached for SW-480 cells after 48 -hours of treatment, but for HT-29 it was 794.84 μg/mL. Moreover, the viability was significantly decreased in a concentration- and time-dependent manner for both cancer cell lines. Examined extracts showed selective inhibitory potential against colon cancer cells. However, after 72 h incubation with CCD 841 CoN cells, the obtained IC50 values for APE and ACE were 594 μg/mL and 709 μg/mL respectively. This suggests that aronia leaves are valuable natural-based products that may support the treatment as chemopreventive agents in colorectal cancer.
本研究旨在探讨梨叶粗酚提取物（ACE）和纯化的富含酚类提取物（APE）对人肠细胞（CCD 841 CoN）和结肠癌细胞（SW-480 和 HT-29）的植物化学组成、抗氧化和细胞毒性潜力。UPLC-Q-TOF-MS 分析证实，梨叶富含结构多样的多酚（ACE 和 APE 分别为 25 和 42 种化合物）。绿原酸和槲皮素-3-鼠李糖苷在两种梨叶提取物中含量最丰富。两种提取物中检测到的多酚总量差异显著，范围从 32.8mg/g（ACE）到 436.3mg/g（APE）。通过体外抗氧化和细胞毒性测定证实了梨叶提取物的生物学潜力。抗氧化活性（ABTS 和 FRAP）的结果表明，APE 表现出比 ACE 强 2 倍的抗氧化特性。APE 对 SW-480 和 HT-29 细胞的细胞毒性作用强于 ACE（MTT 试验）。孵育 48 小时后，发现 APE 以 194.35μg/mL 的浓度抑制 SW-480 细胞生长 50%，而对 HT-29 细胞则为 552.02μg/mL。对于 ACE，在 48 小时处理后，SW-480 细胞的 IC 尚未达到，但对于 HT-29 细胞，IC 为 794.84μg/mL。此外，两种癌细胞系的细胞活力均呈浓度和时间依赖性显著降低。研究中提取的两种提取物对结肠癌细胞具有选择性抑制潜力。然而，在用 CCD 841 CoN 细胞孵育 72 小时后，APE 和 ACE 的 IC50 值分别为 594μg/mL 和 709μg/mL。这表明梨叶是有价值的天然产品，可作为结直肠癌的化学预防剂支持治疗。
