miR-613 通过靶向 LETM1 抑制宫颈癌的进展。
MiR-613 blocked the progression of cervical cancer by targeting LETM1.
机构信息
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China.
出版信息
Eur Rev Med Pharmacol Sci. 2020 Jun;24(12):6576-6582. doi: 10.26355/eurrev_202006_21642.
OBJECTIVE
The purpose of this study was to investigate the potential effects of miR-613 on the development of cervical squamous cell cancer (CSCC) and the relevant mechanism.
PATIENTS AND METHODS
The expression level of miR-613 was detected in CSCC tissues and cells by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Thereafter, online prediction software and Luciferase reporter assays were used to evaluate the possible target of miR-613, and the effects of the miR-613 on SiHa cells were determined in vitro. Then, the changes of protein expression in cells were measured by Western blot. Finally, Cell Counting Kit-8 (CCK-8), wound healing and transwell assays were performed to analyze cell proliferation, invasion, and migration.
RESULTS
It was found that compared with that in normal tissues and cells, miR-613 expression was found suppressed in CSCC tissues and cells. Based on bioinformatics and Luciferase results, miR-613 could target and bind to LETM1. Besides, it was found that miR-613 could regulate the expression of LETM1 in SiHa cells, and miR-613 had a valuable suppressive effect on the proliferation, invasion, and migration of SiHa cells through the subsequent experiments. However, when co-transfection of miR-613 mimics and LV-LETM1 into SiHa cells, the anti-cancer effects of miR-613 on SiHa cells vanished.
CONCLUSIONS
Taken all, this research discovered the function of miR-613 on CSCC by targeting LETM1, revealing that miR-613/LETM1 signal pathway might be a potential therapeutic target for the diagnosis and treatment of CSCC.
目的
本研究旨在探讨 miR-613 对宫颈鳞状细胞癌（CSCC）发展的潜在影响及其相关机制。
方法
采用实时定量聚合酶链反应（qRT-PCR）检测 CSCC 组织和细胞中 miR-613 的表达水平。然后，利用在线预测软件和荧光素酶报告基因实验评估 miR-613 的可能靶标，并在体外研究 miR-613 对 SiHa 细胞的影响。随后，通过 Western blot 检测细胞中蛋白质表达的变化。最后，通过细胞计数试剂盒-8（CCK-8）、划痕愈合和 Transwell 实验分析细胞增殖、侵袭和迁移。
结果
与正常组织和细胞相比，CSCC 组织和细胞中 miR-613 的表达受到抑制。基于生物信息学和荧光素酶结果，miR-613 可以靶向并结合 LETM1。此外，研究发现 miR-613 可以调节 SiHa 细胞中 LETM1 的表达，并且 miR-613 通过随后的实验对 SiHa 细胞的增殖、侵袭和迁移具有有价值的抑制作用。然而，当 miR-613 模拟物和 LV-LETM1 共转染 SiHa 细胞时，miR-613 对 SiHa 细胞的抗癌作用消失。
结论
综上所述，本研究通过靶向 LETM1 发现了 miR-613 对 CSCC 的作用，表明 miR-613/LETM1 信号通路可能是 CSCC 诊断和治疗的潜在治疗靶点。
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