Yang Mingjun, Zhou Wen, Xu Mingming, Han Xiao, Shi Yanyan, Shi Min, Wang Zhipeng
Department of Cardiothoracic Surgery, Affiliated Hospital of Nantong University, No. 20, Xisi Road, Nantong, Jiangsu 226001, China.
Department of Thoracic Surgery, Haimen People's Hospital, No. 1201, Beijing Road, Nantong, Jiangsu 226001, China.
J Oncol. 2023 Feb 16;2023:2311231. doi: 10.1155/2023/2311231. eCollection 2023.
Non-small cell lung cancer (NSCLC) is a crucial crux of cancer-related death, and M2 macrophage polarization facilitates NSCLC development. MicroRNA-613 (miR-613) is a tumor suppressor. This research aimed to clarify the miR-613 function in NSCLC and its impact on M2 macrophage polarization. miR-613 expressions in NSCLC tissues and cells were evaluated using quantitative real-time PCR. For miR-613 function in NSCLC, cell proliferation analysis, cell counting kit-8, flow cytometry, western blot, transwell, and wound-healing were conducted. Meanwhile, the miR-613 impact on M2 macrophage polarization was assessed by the NSCLC models. miR-613 was lessened in NSCLC cells and tissues. It was corroborated that miR-613 overexpression retrained NSCLC cell proliferation, invasion, and migration but facilitated cell apoptosis. Moreover, miR-613 overexpression restrained NSCLC development by repressing M2 macrophage polarization.
Tumor suppressor miR-613 ameliorated NSCLC by restraining M2 macrophage polarization.
非小细胞肺癌（NSCLC）是癌症相关死亡的关键症结所在，M2巨噬细胞极化促进NSCLC发展。微小RNA-613（miR-613）是一种肿瘤抑制因子。本研究旨在阐明miR-613在NSCLC中的功能及其对M2巨噬细胞极化的影响。采用定量实时聚合酶链反应评估NSCLC组织和细胞中miR-613的表达。对于miR-613在NSCLC中的功能，进行了细胞增殖分析、细胞计数试剂盒-8检测、流式细胞术、蛋白质免疫印迹法、Transwell实验和伤口愈合实验。同时，通过NSCLC模型评估miR-613对M2巨噬细胞极化的影响。NSCLC细胞和组织中miR-613表达降低。证实miR-613过表达可抑制NSCLC细胞增殖、侵袭和迁移，但促进细胞凋亡。此外，miR-613过表达通过抑制M2巨噬细胞极化来抑制NSCLC发展。
肿瘤抑制因子miR-613通过抑制M2巨噬细胞极化改善NSCLC。
