Department of Vascular Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Eur Rev Med Pharmacol Sci. 2020 Jun;24(12):7033-7038. doi: 10.26355/eurrev_202006_21696.
The purpose of this study was to uncover the clinical values of serum long non-coding RNA (lncRNA) CCAT2 and miRNA-216b in the properties of carotid atherosclerotic plaques.
Patients with carotid atherosclerotic plaques were assigned into stable plaque group (n=60) and unstable plaque (n=75) group based on their examination results of cervical contrast-enhanced CT examination. Maximal plaque thickness (MAPT) and intima-media thickness (IMT) in each group were determined. Serum levels of lncRNA CCAT2 and miRNA-216b in patients with carotid atherosclerotic plaques were detected by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Moreover, the correlation between serum levels of CCAT2 and miRNA-216b was analyzed by Pearson's correlation analysis. Besides, potential correlations of serum levels of CCAT2 and miRNA-216b with MAPT and IMT in patients with carotid atherosclerotic plaques were assessed as well.
Results revealed that MAPT and IMT were markedly higher in unstable plaque group than those in stable plaque group. Serum level of CCAT2 was higher in unstable plaque group, while miRNA-216b was lower than those in stable group. A negative correlation was identified between serum levels of CCAT2 and miRNA-216b. In addition, CCAT2 level was positively correlated with IMT and MAPT in patients with carotid atherosclerotic plaques, while miRNA-216b level was negatively correlated with them.
Detection of serum levels of CCAT2 and miRNA-216b could be applied for predicting the rupture of carotid atherosclerotic plaques. They may be potential biomarkers predicting ischemic stroke.
本研究旨在揭示血清长链非编码 RNA（lncRNA）CCAT2 和 miRNA-216b 在颈动脉粥样硬化斑块性质中的临床价值。
根据颈增强 CT 检查结果，将颈动脉粥样硬化斑块患者分为稳定斑块组（n=60）和不稳定斑块组（n=75）。测量每组的最大斑块厚度（MAPT）和内膜中层厚度（IMT）。采用逆转录聚合酶链反应（RT-PCR）检测颈动脉粥样硬化斑块患者血清中 lncRNA CCAT2 和 miRNA-216b 的水平。采用 Pearson 相关分析分析血清 CCAT2 和 miRNA-216b 水平之间的相关性。此外，还评估了血清 CCAT2 和 miRNA-216b 水平与颈动脉粥样硬化斑块患者 MAPT 和 IMT 的潜在相关性。
结果显示，不稳定斑块组的 MAPT 和 IMT 明显高于稳定斑块组。不稳定斑块组血清 CCAT2 水平较高，而 miRNA-216b 水平低于稳定组。血清 CCAT2 和 miRNA-216b 水平之间存在负相关。此外，CCAT2 水平与颈动脉粥样硬化斑块患者的 IMT 和 MAPT 呈正相关，而 miRNA-216b 水平与它们呈负相关。
检测血清 CCAT2 和 miRNA-216b 水平可用于预测颈动脉粥样硬化斑块的破裂。它们可能是预测缺血性脑卒中的潜在生物标志物。
