Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Arch Physiol Biochem. 2022 Dec;128(6):1687-1692. doi: 10.1080/13813455.2020.1788606. Epub 2020 Jul 7.
Lipase hydrolyses the ester bonds in triglyceride. It is an important enzyme in medicine and industry. Some pathogen bacteria use this exoenzyme to disrupt the extracellular matrix of host organisms. uses various extracellular enzymes such as lipase to invade its host. In this report, for the first time, bromhexine was introduced as an inhibitor of lipase. Bromhexine is a mucolytic drug which is used in the treatment of respiratory tract disorders. The results showed that bromhexine inhibited the enzyme by competitive inhibition. IC and values of the drug were 0.049 mM and 0.02 mM, respectively. Arrhenius plot showed that the drug reduced the activation energy. The enzyme was purified and SDS-PAGE showed that its molecular weight is 13 kDa. Fluorescence measurement revealed that binding of the drug to lipase could make structural changes in the enzyme. Inhibition of lipase by bromhexine could be applicable in medicine.
脂肪酶水解甘油三酯中的酯键。它是医学和工业中的一种重要酶。一些病原体细菌利用这种外切酶破坏宿主生物的细胞外基质。利用各种细胞外酶，如脂肪酶，入侵其宿主。在本报告中，溴己新首次被引入作为脂肪酶的抑制剂。溴己新是一种黏液溶解药物，用于治疗呼吸道疾病。结果表明，溴己新通过竞争性抑制来抑制该酶。该药物的 IC 和 值分别为 0.049 mM 和 0.02 mM。Arrhenius 图表明该药物降低了活化能。该酶已被纯化，SDS-PAGE 显示其分子量为 13 kDa。荧光测量表明，药物与脂肪酶的结合可以使酶的结构发生变化。溴己新抑制脂肪酶的作用可能适用于医学领域。
