Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-3-11, Takeda, Kofu, Yamanashi, 400-8511, Japan.
J Mass Spectrom. 2021 Apr;56(4):e4583. doi: 10.1002/jms.4583. Epub 2020 Jul 7.
The performance of a compact high-pressure electrospray ionization (HP-ESI) source that can be readily used for commercial atmospheric pressure ionization (API) mass spectrometers is reported. The ion source employs a converging-diverging outlet nozzle, and ions/droplets generated inside the high-pressure compartment are carried by the high-velocity air jet toward the mass spectrometry (MS) ion inlet placed under the atmospheric pressure. With the use of a shielding electrode, the HP-ESI can also be operated with its emitter held at ground potential. This feature prevents the flow of current from the emitter to other electrically grounded components and facilitates the connection of ion source to liquid chromatography (LC) columns or capillary electrophoresis. Sensitive detection of proteins from highly conductive aqueous solutions such as 0.1% trifluoroacetic acid (TFA) solution and the prevention of electrochemical artifacts by the grounded emitter operation are demonstrated.
本文报道了一种紧凑式高压电喷雾电离（HP-ESI）源的性能，该源可方便地用于商业大气压电离（API）质谱仪。该离子源采用收敛-发散出口喷嘴，高压腔内产生的离子/液滴由高速气流携带朝向置于大气压下的质谱（MS）离子入口。通过使用屏蔽电极，HP-ESI 也可以在发射极接地电位下运行。此功能可防止电流从发射极流向其他电接地部件，并便于将离子源连接到液相色谱（LC）柱或毛细管电泳。通过接地发射极操作，实现了对高导电性水溶液（如 0.1%三氟乙酸（TFA）溶液）中蛋白质的灵敏检测，并防止了电化学伪影的产生。
