Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan.
J Am Soc Mass Spectrom. 2020 May 6;31(5):1015-1018. doi: 10.1021/jasms.0c00052. Epub 2020 Apr 6.
A new high-pressure ESI source that can be readily used for commercial API mass spectrometers in a plug-and-play manner without any modification on the ion sampling interface is introduced. The emitter can be operated at ground potential, and the positive mode electrospray is generated by applying a negative high potential to the counter electrode. A shielding electrode effectively shields the opposing electric field and improves the ion transmission. This feature facilitates the direct connection of the ESI emitter to the electrically grounded components. The application of the present ion source to the high-temperature (>100 °C) capillary liquid chromatography for high-speed separation of peptide and proteins is demonstrated using a monolithic polymeric column.
介绍了一种新的高压电喷雾源，可以直接用于商业 API 质谱仪，无需对离子采样接口进行任何修改，即插即用。发射器可以工作在接地电位，通过向对电极施加负高电压来产生正模式电喷雾。屏蔽电极有效地屏蔽了反向电场，提高了离子传输效率。这一特点使得电喷雾发射器能够直接连接到电气接地部件。本离子源在高温（>100°C）毛细管液相色谱中用于肽和蛋白质的高速分离，采用整体聚合物柱进行了演示。
