Wu Jian-Yi, Gu Li, Hua Zu-Lin, Li Xiao-Qing, Lu Ying, Chu Ke-Jian
Key Laboratory of Integrated Regulation and Resource Development on Shallow Lake of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China; National Engineering Research Center of Water Resources Efficient Utilization and Engineering Safety, Hohai University, Nanjing 210098, China.
Key Laboratory of Integrated Regulation and Resource Development on Shallow Lake of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China; National Engineering Research Center of Water Resources Efficient Utilization and Engineering Safety, Hohai University, Nanjing 210098, China.
J Hazard Mater. 2021 Jan 5;401:123252. doi: 10.1016/j.jhazmat.2020.123252. Epub 2020 Jun 21.
Determination of the effects of Escherichia coli (E. coli) pollution on agricultural pond ecosystems with vegetation at different life stages is essential for the protection of ecological functions. However, no comprehensive study has yet shown the responses of epiphytic microbial communities to E. coli invasion during plant decay. Thus, this study was conducted to clarify variation in the decay of the following aquatic plants-Myriophyllum aquaticum, Nymphaea tetragona and Phragmites australis after E. coli pollution. Exogenous E. coli especially shifted the epiphytic microbial composition and distribution of P. australis. Stronger effects of E. coli on the archaeal community (edges/nodes = 0.818 < 1, modularity = 0.654; lower clustered structure, 0.389) were found than on the bacterial community (edges/nodes = 1.538 > 1, modularity = 1.291 > 0.654; higher clustered, 0.593). During plant decomposition, E. coli weakened methanogenesis by regulating the network of core genera Methanobacterium and Methanospirillum (spearman, P < 0.05), stimulated the accumulation of organic matters in water (P < 0.05). Similarly, nitrification and denitrification increased and decreased through network regulation in relative biomass of genera Devosia and Desulfovibrio (P < 0.05), respectively. The results provided theoretical supports for eutrophication management in pond ecosystems threatened by E. coli pollution.
确定大肠杆菌污染对处于不同生命阶段的带植被农业池塘生态系统的影响，对于保护生态功能至关重要。然而，尚无全面研究表明附生微生物群落对植物腐烂过程中大肠杆菌入侵的反应。因此，本研究旨在阐明大肠杆菌污染后以下水生植物——水蕴草、睡莲和芦苇腐烂情况的变化。外源大肠杆菌尤其改变了芦苇的附生微生物组成和分布。发现大肠杆菌对古菌群落的影响更强（边/节点 = 0.818 < 1，模块度 = 0.654；较低的聚类结构，0.389），而对细菌群落的影响较弱（边/节点 = 1.538 > 1，模块度 = 1.291 > 0.654；较高的聚类，0.593）。在植物分解过程中，大肠杆菌通过调节核心属甲烷杆菌属和甲烷螺菌属的网络来削弱甲烷生成（斯皮尔曼，P < 0.05），刺激水中有机物的积累（P < 0.05）。同样，硝化作用和反硝化作用分别通过对德沃斯氏菌属和脱硫弧菌属相对生物量的网络调节而增加和减少（P < 0.05）。这些结果为受大肠杆菌污染威胁的池塘生态系统富营养化管理提供了理论支持。
