Department of Pharmacology, Qazvin University of Medical Sciences, Qazvin, Iran.
Department of Pathology, Oncology Research Center, Iran University of Medical Sciences, Tehran, Iran.
Cells Tissues Organs. 2020;209(1):13-25. doi: 10.1159/000507790. Epub 2020 Jul 7.
Due to their ability in self-renewing and differentiation into a wide variety of tissues, mesenchymal stem cells (MSCs) exhibit outstanding potential for regenerative medicine. This study was aimed at investigating different aspects of MSC therapy in controlling hyperglycemia in streptozotocin-induced diabetes rats. Using an islet cell differentiation protocol, bone marrow (BM) MSCs were differentiated into insulin-producing cells (IPCs). The differentiation process was evaluated by immunocytochemistry, reverse transcriptase PCR, and dithizone staining. Diabetic animals in 4 diabetic individual groups received normal saline, BM-MSCs, coadministration of BM-MSCs with supernatant, and IPCs. Blood glucose and insulin levels were monitored during the experiment. Immunohistochemical analysis of the pancreas was performed at the end of the experiment. Administration of BM-MSCs could not reverse glucose and insulin levels in experimental animals as efficiently as cotransplantation of BM-MSCs with supernatant. The effect of coadministration of BM-MSCs with supernatant and transplantation of IPCs on controlling hyperglycemia is comparable. Immunohistochemical analysis showed that number and size of islets per section were significantly increased in groups receiving IPCs and BM-MSC-supernatant compared to the MSC group of animals. In conclusion, coadministration of BM-MSCs with supernatant could be used as efficiently as IPC transplantation in controlling hyperglycemia in diabetic rats.
由于间充质干细胞（MSCs）具有自我更新和分化为多种组织的能力，因此它们在再生医学中具有突出的潜力。本研究旨在探讨 MSC 治疗在控制链脲佐菌素诱导的糖尿病大鼠高血糖方面的不同方面。使用胰岛细胞分化方案，将骨髓（BM）MSCs 分化为产生胰岛素的细胞（IPCs）。通过免疫细胞化学、逆转录 PCR 和二硫腙染色评估分化过程。将 4 只糖尿病动物分别分为生理盐水组、BM-MSCs 组、BM-MSCs 与上清液共给药组和 IPCs 组，在实验过程中监测血糖和胰岛素水平。实验结束时对胰腺进行免疫组织化学分析。与共移植 BM-MSCs 与上清液相比，BM-MSCs 的给药并不能像有效控制实验动物的葡萄糖和胰岛素水平。共给予 BM-MSCs 与上清液和移植 IPCs 对控制高血糖的效果相当。免疫组织化学分析显示，与 MSC 组动物相比，接受 IPCs 和 BM-MSC 上清液的组中每个切片的胰岛数量和大小明显增加。总之，共给予 BM-MSCs 与上清液与移植 IPCs 一样有效地控制糖尿病大鼠的高血糖。
