Department of Histology and Embryology, Faculty of Medicine, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Karłowicza 24, 85-092 Bydgoszcz, Poland.
Molecules. 2020 Jul 3;25(13):3045. doi: 10.3390/molecules25133045.
Cancers are one of the leading causes of deaths nowadays. The development of new treatment schemes for oncological diseases is an interesting direction in experimental medicine. Therefore, the evaluation of the influence of two alkaloids-piperlongumine (PL), sanguinarine (SAN) and their combination-on the basic life processes of the A549 cell line was considered reasonable.
The aim was achieved by analyzing the cytotoxic effects of PL and SAN and their combination in the ratio of 4:1 on the induction of cell death, changes in the distribution of cell cycle phases, reorganization of cytoskeleton and metastatic potential of A549 cells. The versatility of the applied concentration ratio was evaluated in terms of other cancer cell lines: MCF-7, H1299 and HepG2.
The results obtained from the MTT assay indicated that the interaction between the alkaloids depends on the concentration and type of cells. Additionally, the compounds and their combination did not exhibit a cytotoxic effect against normal cells. The combined effects of PL and SAN increased apoptosis and favored metastasis inhibition.
Selected alkaloids exhibit a cytotoxic effect on A549 cells. In turn, treatment with the combination of PL and SAN in a 4:1 ratio indicates a synergistic effect and is associated with an increase in the level of reactive oxygen species (ROS).
癌症是当今主要的死亡原因之一。开发治疗肿瘤疾病的新方案是实验医学中一个很有趣的方向。因此，评估两种生物碱——胡椒碱（PL）、血根碱（SAN）及其组合对 A549 细胞系基本生命过程的影响是合理的。
通过分析 PL 和 SAN 及其组合在 4:1 比例下对诱导细胞死亡、细胞周期各阶段分布变化、细胞骨架重排和 A549 细胞转移潜能的影响，实现了这一目标。应用浓度比的多功能性还在其他癌细胞系 MCF-7、H1299 和 HepG2 中进行了评估。
MTT 检测结果表明，生物碱之间的相互作用取决于浓度和细胞类型。此外，这些化合物及其组合对正常细胞没有细胞毒性作用。PL 和 SAN 的联合作用增加了细胞凋亡，有利于抑制转移。
所选生物碱对 A549 细胞具有细胞毒性作用。相反，以 4:1 的比例联合使用 PL 和 SAN 治疗表现出协同作用，并与活性氧（ROS）水平的升高有关。
