Hałas-Wiśniewska Marta, Arendt Wioletta, Grzanka Alina, Izdebska Magdalena
Department of Histology and Embryology, Faculty of Medicine, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Karłowicza 24, 85-092 Bydgoszcz, Poland.
Pharmaceuticals (Basel). 2024 Dec 24;18(1):3. doi: 10.3390/ph18010003.
The literature reports that ezrin (EZR) is important as a linker between microfilaments and cellular environments. Moreover, it affects cancer cell migration, but the exact mechanism is not fully understood. In this study, we aimed to investigate the role of EZR in the migration of two different types of cervical cancer cells-from primary lesion (SiHa) and lymph node metastases (HT-3). In addition, we showed for the first time that a reduced EZR protein level affects the cellular response to the routinely used treatment with cisplatin.
The most important stage of the study consisted of conducting a series of tests enabling the assessment of the migration potential of cervical cancer cells without altered EZR expression and with silenced protein expression.
Reducing the EZR level resulted in a decrease in the invasive and migration potential of SiHa and HT-3 cells' inhibition of colony formation, a decrease in adhesive properties, and a strong reorganization of F-actin with a dominance of cells with a mitotic catastrophe phenotype. A lower level of protein significantly reduces the motor skills of SiHa and HT-3 cervical cancer cells.
This significantly affects the assessment of EZR as a potential factor that can limit the development of metastases in targeted cancer therapy of cervical cancer.
文献报道埃兹蛋白（EZR）作为微丝与细胞环境之间的连接蛋白具有重要作用。此外，它影响癌细胞迁移，但其确切机制尚未完全明确。在本研究中，我们旨在探究EZR在两种不同类型宫颈癌细胞（来自原发性病变的SiHa细胞和淋巴结转移灶的HT - 3细胞）迁移中的作用。此外，我们首次表明EZR蛋白水平降低会影响细胞对常规使用的顺铂治疗的反应。
该研究最重要的阶段包括进行一系列测试，以评估EZR表达未改变以及蛋白表达沉默的宫颈癌细胞的迁移潜能。
降低EZR水平导致SiHa和HT - 3细胞的侵袭和迁移潜能降低、集落形成受到抑制、黏附特性下降，以及F - 肌动蛋白发生强烈重组，具有有丝分裂灾难表型的细胞占优势。较低的蛋白水平显著降低了SiHa和HT - 3宫颈癌细胞的运动能力。
这对将EZR评估为宫颈癌靶向癌症治疗中可限制转移发展的潜在因素具有重要影响。
