Ruta Claudia, De Mastro Giuseppe, Ancona Simona, Tagarelli Anna, De Cillis Francesca, Benelli Carla, Lambardi Maurizio
Department of Agricultural and Environmental Science, Università degli Studi di Bari Aldo Moro, via Amendola 165/a, 70125 Bari, Italy.
IBE-Institute of BioEconomy, National Research Council (CNR), 50019 Sesto Fiorentino (Florence), Italy.
Plants (Basel). 2020 Jul 4;9(7):844. doi: 10.3390/plants9070844.
Goji ( L.) has recognized nutritive and antioxidant properties and many products are commercialized for health in food market. Besides its food use, goji has been the subject of more than 2000 years of traditional Chinese medicine, using berries, root bark, and leaves. Here, the potential of the liquid culture in temporary immersion system (TIS) by using the bioreactor Plantform was tested for the large-scale production of high-quality goji shoots and the subsequent production of total phenols and flavonoids. The three tested immersion cycles differently influenced the shoot quality in terms of proliferation and hyperhydricity. The best immersion cycle (time and frequency) was proven to have the shortest daily immersion time (6 min every 24 h) which ensured good levels of relative growth and multiplication rate, very limited onset of hyperydricity, and the longest shoots, promoting direct rooting after only 30 days of culture. In comparison with the semisolid culture, the TIS culture resulted in an increase of the total phenolic content (TPC) and in a lower value of the total flavonoid content (TFC). However, considering the higher quantity of biomass produced in the Plantform bioreactor, the difference in terms of TFC productivity between semisolid medium and TIS liquid culture was proven to be statistically equivalent.
枸杞（Lycium barbarum L.）具有公认的营养和抗氧化特性，许多产品在食品市场上作为保健品商业化销售。除了用于食品外，枸杞作为中药材已有2000多年的历史，其果实、根皮和叶子均可入药。在此，我们测试了使用生物反应器Plantform的临时浸没系统（TIS）进行液体培养，以大规模生产高质量枸杞嫩枝，并随后生产总酚和黄酮类化合物的潜力。三个测试的浸没周期在增殖和玻璃化方面对嫩枝质量有不同的影响。最佳浸没周期（时间和频率）被证明具有最短的每日浸没时间（每24小时6分钟），这确保了良好的相对生长水平和繁殖率，玻璃化现象的发生非常有限，并且嫩枝最长，仅培养30天后就能促进直接生根。与半固体培养相比，TIS培养导致总酚含量（TPC）增加，总黄酮含量（TFC）值降低。然而，考虑到在Plantform生物反应器中产生的生物量更多，半固体培养基和TIS液体培养之间在TFC生产力方面的差异被证明在统计学上是等效的。
