Balle Christina, Esra Rachel, Havyarimana Enock, Jaumdally Shameem Z, Lennard Katie, Konstantinus Iyaloo N, Barnabas Shaun L, Happel Anna-Ursula, Gill Katherine, Pidwell Tanya, Lingappa Jairam R, Gamieldien Hoyam, Bekker Linda-Gail, Passmore Jo-Ann S, Jaspan Heather B
Department of Pathology, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, 7925 Cape Town, South Africa.
Desmond Tutu HIV Centre, University of Cape Town, 7925 Cape Town, South Africa.
Microorganisms. 2020 Jul 4;8(7):1004. doi: 10.3390/microorganisms8071004.
Bacterial vaginosis (BV) and periodontal disease (PD) are characterised as bacterial dysbioses. Both are associated with an increased risk of poor pregnancy outcomes, yet it is unknown whether PD and BV are related. We characterised the oral microbiota of young South African females with a high prevalence of BV and investigated the association between oral communities and vaginal microbiota. DNA was extracted from vaginal lateral wall, saliva and supragingival plaque samples from 94 adolescent females (aged 15-19 years). 16S rRNA gene sequencing of the V4 hypervariable region was performed for analysis of the oral and vaginal microbiota and BV status was determined by Nugent scoring. The core oral microbiota was predominately comprised of Firmicutes followed by Proteobacteria and Bacteroidetes. The salivary microbiota of participants with BV was more diverse than those with lactobacillus-dominated communities ( = 0.030). PD-associated bacterial species, including and were enriched in the supragingival microbiota of women with non-optimal vaginal communities compared to those with -dominant communities, while and were enriched in the saliva of women with non-optimal vaginal microbiota. These data suggest a relationship between oral and vaginal dysbiosis, warranting further investigation into whether they are casually related.
细菌性阴道病（BV）和牙周病（PD）的特征为细菌群落失调。两者均与不良妊娠结局风险增加相关，但PD和BV是否相关尚不清楚。我们对BV患病率高的南非年轻女性的口腔微生物群进行了特征分析，并研究了口腔群落与阴道微生物群之间的关联。从94名青春期女性（年龄15 - 19岁）的阴道侧壁、唾液和龈上菌斑样本中提取DNA。对V4高变区进行16S rRNA基因测序，以分析口腔和阴道微生物群，并通过纽金特评分确定BV状态。核心口腔微生物群主要由厚壁菌门组成，其次是变形菌门和拟杆菌门。BV患者的唾液微生物群比以乳酸杆菌为主的群落的患者更为多样（P = 0.030）。与以乳酸杆菌为主的群落的女性相比，与PD相关的细菌种类，包括[具体细菌名称1]和[具体细菌名称2]，在阴道群落不理想的女性的龈上微生物群中更为丰富，而[具体细菌名称3]和[具体细菌名称4]在阴道微生物群不理想的女性的唾液中更为丰富。这些数据表明口腔和阴道失调之间存在关联，值得进一步研究它们是否存在因果关系。
