Hassuna Noha A, AbdelAziz Reem A, Zakaria Amira, Abdelhakeem Mohammed
Department of Medical Microbiology and Immunology, Faculty of Medicine, Minia University, Minia, Egypt.
Department of Pediatrics, Faculty of Medicine, Minia University, Minia, Egypt.
Front Microbiol. 2020 Jun 19;11:1375. doi: 10.3389/fmicb.2020.01375. eCollection 2020.
Neonatal sepsis is a nuisance to clinicians and medical microbiologists, particularly those cases caused by . Thus, we aimed at investigating the profile and mechanisms of antibiotic resistance and the clonal relationships between isolated from neonates at the largest tertiary care hospital's neonatal intensive care units (NICUs) in Minia, Egypt.
This study comprised 156 neonates diagnosed with culture-proven sepsis from February 2019 to September 2019, at a major NICU of Minia City. All isolates were collected and characterized by antimicrobial profile, resistance genotype, and pulsed-field gel electrophoresis typing.
Twenty-four isolates (15.3%) were collected out of the 156 sepsis diagnosed neonates. These samples showed extensive drug resistance (XDR) to most of the tested antimicrobials, except fluoroquinolones. All the isolates possessed and carbapenemase genes, while gene was detected in 95.8%. Considering extended-spectrum β-lactamases genes, was found in all the isolates and gene in 75% of them. The plasmid-mediated quinolone resistance gene S, was predominantly found among our isolates in comparison to B or A. A moderate degree of clonal relatedness was observed between the isolates.
To the best of our knowledge, this the first report of an alarming occurrence of XDR among isolates recovered from neonatal sepsis in Egypt. Our data necessitate proper antimicrobial stewardship as the choices will be very limited.
新生儿败血症对临床医生和医学微生物学家来说是个麻烦事，尤其是由……引起的那些病例。因此，我们旨在调查埃及米尼亚最大的三级护理医院新生儿重症监护病房（NICUs）中从新生儿分离出的……的抗生素耐药性特征和机制以及克隆关系。
本研究纳入了2019年2月至2019年9月在米尼亚市一家主要的新生儿重症监护病房被诊断为血培养确诊败血症的156名新生儿。收集所有……分离株，并通过抗菌谱、耐药基因型和脉冲场凝胶电泳分型进行鉴定。
在156名被诊断为败血症的新生儿中收集到24株……分离株（15.3%）。这些样本对大多数测试抗菌药物表现出广泛耐药（XDR），氟喹诺酮类药物除外。所有……分离株都具有……和……碳青霉烯酶基因，而……基因在95.8%的分离株中被检测到。考虑到超广谱β-内酰胺酶基因，所有分离株中都发现了……，75%的分离株中发现了……基因。与B或A相比，质粒介导的喹诺酮耐药基因S在我们的分离株中占主导地位。分离株之间观察到中等程度的克隆相关性。
据我们所知，这是埃及首次报道从新生儿败血症中分离出的……出现令人担忧的广泛耐药情况。我们的数据表明需要进行适当的抗菌药物管理，因为选择将非常有限。
