Shay Elizabeth, Sangwan Naseer, Padmanabhan Roshan, Lundy Scott, Burkey Brian, Eng Charis
Genomic Medicine Institute, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA.
Cleveland Clinic Lerner College of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
Oncotarget. 2020 Jun 23;11(25):2375-2386. doi: 10.18632/oncotarget.27629.
The etiology of head and neck squamous cell carcinoma (HNSCC) is not fully understood. While risk factors such as positive human papilloma virus (HPV) status, smoking and tobacco use have been identified, they do not account for all cases of the disease. We aimed to characterize the bacteriome, mycobiome and mycobiome-bacteriome interactions of oral wash in HNSCC patients and to determine if they are distinct from those of the oral wash of matched non-HNSCC patients. Oral wash samples were collected from 46 individuals with HNSCC and 46 controls for microbiome analyses. We identified three fungal phyla and eleven bacterial phyla of which Ascomycota (fungi, 72%) and Firmicutes (bacteria, 39%) were the most dominant, respectively. A number of organisms were identified as being differentially abundant between oral wash samples from patients with HNSCC and oral wash samples from those without HNSCC. Of note, strains of and were differentially abundant and was depleted in those with HNSCC compared to oral wash from those without HNSCC. Our results suggest that the oral cavity of HNSCC patients harbors unique differences in the mycobiome, bacteriome, and microbiome interactions when compared to those of control patients.
头颈部鳞状细胞癌（HNSCC）的病因尚未完全明确。虽然已确定了一些风险因素，如人乳头瘤病毒（HPV）阳性状态、吸烟和使用烟草，但这些因素并不能解释所有该疾病病例。我们旨在表征HNSCC患者口腔冲洗液中的细菌群落、真菌群落以及真菌群落与细菌群落的相互作用，并确定它们是否与匹配的非HNSCC患者口腔冲洗液中的不同。从46例HNSCC患者和46例对照者中收集口腔冲洗液样本进行微生物组分析。我们鉴定出三个真菌门和十一个细菌门，其中子囊菌门（真菌，72%）和厚壁菌门（细菌，39%）分别最为占主导地位。在HNSCC患者的口腔冲洗液样本和非HNSCC患者的口腔冲洗液样本之间，鉴定出许多丰度存在差异的生物体。值得注意的是，与非HNSCC患者的口腔冲洗液相比，HNSCC患者的口腔冲洗液中 和 的菌株丰度存在差异，且 减少。我们的结果表明，与对照患者相比，HNSCC患者口腔中的真菌群落、细菌群落及微生物组相互作用存在独特差异。
