Department of Microbiology and Infectious Diseases, Toho University Graduate School of Medicine, 5-21-16 Omorinishi, Ota-ku, Tokyo, 143-8540, Japan.
Department of Infectious Diseases, Toho University Omori Medical Center, 6-11-1 Omorinishi, Ota-ku, Tokyo, 143-8541, Japan.
BMC Microbiol. 2018 Oct 5;18(1):127. doi: 10.1186/s12866-018-1274-9.
Microbial flora in several organs of HIV-infected individuals have been characterized; however, the palatine tonsil bacteriome and mycobiome and their relationship with each other remain unclear. Determining the palatine tonsil microbiome may provide a better understanding of the pathogenesis of oral and systemic complications in HIV-infected individuals. We conducted a cross-sectional study to characterize the palatine tonsil microbiome in HIV-infected individuals.
Palatine tonsillar swabs were collected from 46 HIV-infected and 20 HIV-uninfected individuals. The bacteriome and mycobiome were analyzed by amplicon sequencing using Illumina MiSeq. The palatine tonsil bacteriome of the HIV-infected individuals differed from that of HIV-uninfected individuals in terms of the decreased relative abundances of the commensal genera Neisseria and Haemophilus. At the species level, the relative abundances and presence of Capnocytophaga ochracea, Neisseria cinerea, and Selenomonas noxia were higher in the HIV-infected group than those in the HIV-uninfected group. In contrast, fungal diversity and composition did not differ significantly between the two groups. Microbial intercorrelation analysis revealed that Candida and Neisseria were negatively correlated with each other in the HIV-infected group. HIV immune status did not influence the palatine tonsil microbiome in the HIV-infected individuals.
HIV-infected individuals exhibit dysbiotic changes in their palatine tonsil bacteriome, independent of immunological status.
已对 HIV 感染者的多个器官中的微生物菌群进行了描述；然而，尚不清楚腭扁桃体的细菌组和真菌组及其相互关系。确定腭扁桃体微生物组可能有助于更好地了解 HIV 感染者口腔和全身并发症的发病机制。我们进行了一项横断面研究，以描述 HIV 感染者的腭扁桃体微生物组。
从 46 名 HIV 感染者和 20 名 HIV 未感染者中采集了腭扁桃体拭子。使用 Illumina MiSeq 通过扩增子测序分析细菌组和真菌组。与 HIV 未感染者相比，HIV 感染者的腭扁桃体细菌组中共生属奈瑟菌和嗜血杆菌的相对丰度降低。在种水平上，HIV 感染者组中 Capnocytophaga ochracea、Neisseria cinerea 和 Selenomonas noxia 的相对丰度和存在更为常见。相比之下，两组之间的真菌多样性和组成没有显著差异。微生物相互关联分析显示，在 HIV 感染者组中，念珠菌和奈瑟菌相互呈负相关。HIV 免疫状态并未影响 HIV 感染者的腭扁桃体微生物组。
HIV 感染者的腭扁桃体细菌组存在失调变化，而与免疫状态无关。
