AL 721：一种强效的人类T淋巴细胞膜调节剂？

AL 721: a potent membrane modulating agent of human T-lymphocytes?

作者信息

Offner F A, Winter U

机构信息

Institute for General and Experimental Pathology, Medical School, University of Innsbruck.

出版信息

Wien Klin Wochenschr. 1988 Sep 9;100(17):597-9.

PMID:3263733

Abstract

This report deals with AL 721, a reputedly potent membrane fluidizing agent. A correlation has been sought between the effects of AL 721 on membrane fluidity and mitogen responsiveness of human peripheral blood lymphocytes. The AL 721-induced increase in membrane "fluidity", as measured by fluorescence polarisation with 1,6-diphenyl-1,3,5-hexatriene, was found to be an artefact arising from lipid uptake by monocytes. Mitogen responses were enhanced by inclusion of AL 721 in the serum-free culture medium. However, this observation is more likely explained by provision of lymphocytes with nutritional lipids required for optimal growth in vitro, than altered responsiveness of "fluidized" lymphocytes.

摘要

本报告涉及AL 721，一种据称有效的膜流化剂。人们一直在寻找AL 721对人外周血淋巴细胞膜流动性和丝裂原反应性的影响之间的相关性。通过用1,6-二苯基-1,3,5-己三烯进行荧光偏振测量发现，AL 721诱导的膜“流动性”增加是单核细胞摄取脂质引起的假象。在无血清培养基中加入AL 721可增强丝裂原反应。然而，这一观察结果更可能是由于为淋巴细胞提供了体外最佳生长所需的营养脂质，而不是“流化”淋巴细胞的反应性改变。