Duong Thuy-Yen, Tan Mun Hua, Lee Yin Peng, Croft Larry, Austin Christopher M
College of Aquaculture and Fisheries, Can Tho University, Can Tho City, Viet Nam.
Deakin Genomics Centre, Deakin University, Geelong 3220, Victoria, Australia.
Data Brief. 2020 Jun 16;31:105861. doi: 10.1016/j.dib.2020.105861. eCollection 2020 Aug.
Freshwater catfish of the genus , known as the airbreathing catfish, are widespread and important for food security through small scale inland fisheries and aquaculture. Limited genomic data are available for this important group of fishes. The bighead catfish () is a commercial aquaculture species in southeast Asia used for aquaculture and threatened in its natural environment through habitat destruction, over-exploitation and competition from other introduced species of . Despite its commercial importance and threats to natural populations, public databases do not include any genomic data for . We present the first genomic data for the bighead catfish from Illumina sequencing. A total of 128 Gb of sequence data in paired-end 150 bp reads were assembled , generating a final assembly of 883 Mbp contained in 27,833 scaffolds (N length: 80.8 kbp) with BUSCO completeness assessments of 96.3% and 87.6% based on metazoan and Actinopterygii ortholog datasets, respectively. Annotation of the genome predicted 21,124 gene sequences, which were assigned putative functions based on homology to existing protein sequences in public databases. Raw fastq reads and the final version of the genome assembly have been deposited in the NCBI (BioProject: PRJNA604477, WGS: JAAGKR000000000, SRA: SRR11188453). The complete mitochondrial genome was also recovered from the same sequence read dataset and is available on NCBI (accession: MT109097), representing the first mitogenome for this species. Lastly, we find an expansion of the and genes thought to be associated with adaptations to air-breathing and a semi-terrestrial life style in this genus of catfish.
属的淡水鲶鱼,即所谓的呼吸空气的鲶鱼,分布广泛,通过小规模内陆渔业和水产养殖对粮食安全具有重要意义。对于这一重要鱼类群体,可用的基因组数据有限。大头鲶()是东南亚的一种商业养殖鱼类,用于水产养殖,但由于栖息地破坏、过度开发以及来自其他引入的物种的竞争,其在自然环境中受到威胁。尽管它具有商业重要性且对自然种群构成威胁,但公共数据库中没有该物种的任何基因组数据。我们展示了通过Illumina测序获得的大头鲶的首批基因组数据。对成对末端150 bp读段中的总共128 Gb序列数据进行了组装,生成了一个最终组装体,大小为883 Mbp,包含在27,833个支架中(N50长度:80.8 kbp),基于后生动物和辐鳍鱼直系同源数据集的BUSCO完整性评估分别为96.3%和87.6%。对基因组的注释预测了21,124个基因序列,这些序列基于与公共数据库中现有蛋白质序列的同源性被赋予了假定功能。原始的fastq读段和基因组组装的最终版本已存入NCBI(生物项目:PRJNA604477,全基因组枪击测序:JAAGKR000000000,序列读取档案:SRR11188453)。完整的线粒体基因组也从相同的序列读取数据集中获得,并可在NCBI上获取(登录号:MT109097),这是该物种的首个线粒体基因组。最后,我们发现该属鲶鱼中与适应呼吸空气和半陆地生活方式相关的基因和基因有所扩增。