Martín-Cano Francisco E, Gaitskell-Phillips Gemma, Ortiz-Rodríguez José M, Silva Antonio, Gil Cruz, Ortega-Ferrusola Cristina, Peña Fernando J
Laboratory of Equine Reproduction and Spermatology, Department of Animal Medicine, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
SIPA University of Extremadura, Cáceres, Spain.
Data Brief. 2020 Jun 20;31:105887. doi: 10.1016/j.dib.2020.105887. eCollection 2020 Aug.
This paper provides the dataset of proteins of stallion ejaculates before and after cryopreservation. The data report the analysis and identification of stallion sperm proteins obtained from the same ejaculates and split in two subsamples. The first aliquot consisted on fresh spermatozoa and the second aliquot was frozen and thawed spermatozoa. Samples were analyzed using a UHPLC/MS/MS system consisting of an Agilent 1290 infinity series UHPLC coupled to an Agilent 6550 Q-TOF mass spectrometer (Agilent Technologies, Santa Clara, CA, USA). We provide a data set of 2226 different proteins, with 2180 aligned to the equine proteome database. The data can be used to identify potential targets to be explored to improve techniques for cryopreservation of spermatozoa. This data article refers to the article "Proteomic profiling of stallion spermatozoa suggests changes in sperm metabolism and compromised redox regulation after cryopreservation" (Martín Cano et al.; 2020) [1].
本文提供了种马精液冷冻保存前后的蛋白质数据集。数据报告了从同一射精样本中获取并分成两个子样本的种马精子蛋白质的分析和鉴定结果。第一份等分试样由新鲜精子组成，第二份等分试样是冷冻和解冻后的精子。使用由安捷伦1290无限系列超高效液相色谱仪与安捷伦6550 Q-TOF质谱仪联用的超高效液相色谱/串联质谱系统（美国加利福尼亚州圣克拉拉市安捷伦科技公司）对样本进行分析。我们提供了一个包含2226种不同蛋白质的数据集，其中2180种与马蛋白质组数据库比对。这些数据可用于识别潜在靶点，以探索改进精子冷冻保存技术。本数据文章引用了“种马精子的蛋白质组学分析表明冷冻保存后精子代谢变化及氧化还原调节受损”（马丁·卡诺等人；2020年）[1]这篇文章。
