Department of Microbiology, Harbin Medical University, Harbin, China.
Wu Lien-Teh Institute, Harbin Medical University, Harbin, China.
Int J Cancer. 2020 Nov 15;147(10):2879-2890. doi: 10.1002/ijc.33192. Epub 2020 Jul 31.
Laryngeal squamous cell carcinoma (LSCC) is a highly malignant tumor originated from respiratory system. Although there have been many improvements in therapy until now, reducing the high mortality remains difficult. Understanding the cellular heterogeneity of LSCC could contribute to improve this problem. Single-cell RNA sequencing was applied to dissect the cell composition and molecular characteristics of LSCC tissues. Immunohistochemistry staining of the LSCC tissues was performed to identify the spatial location of tumor cells. Survival analysis of marker genes was executed in The Cancer Genome Atlas to verify the correlation between each cell clusters and patients' prognosis. The LSCC tissue cells were finely grouped into various clusters, including tumor cells, immune cells, epithelial cells, fibroblasts and endothelial cells. Notably, in tumor cells, keratinocyte-like cells were in the core of tumor while malignant proliferating cells were located at the tumor edge. The malignant proliferating cells were correlated with poor prognosis. In summary, this is the first study to delineate a landscape of the LSCC intratumor heterogeneity. Our work might help researchers have a better understanding for tumor progression.
喉鳞状细胞癌 (LSCC) 是一种起源于呼吸系统的高度恶性肿瘤。尽管目前在治疗方面已经有了很多进展，但降低高死亡率仍然很困难。了解 LSCC 的细胞异质性有助于改善这一问题。单细胞 RNA 测序被应用于剖析 LSCC 组织的细胞组成和分子特征。对 LSCC 组织进行免疫组织化学染色，以确定肿瘤细胞的空间位置。在癌症基因组图谱中对标记基因进行生存分析，以验证每个细胞簇与患者预后之间的相关性。LSCC 组织细胞被精细地分为各种簇，包括肿瘤细胞、免疫细胞、上皮细胞、成纤维细胞和内皮细胞。值得注意的是，在肿瘤细胞中，角蛋白细胞样细胞位于肿瘤核心，而恶性增殖细胞位于肿瘤边缘。恶性增殖细胞与预后不良相关。总之，这是首次描绘 LSCC 肿瘤内异质性的研究。我们的工作可能有助于研究人员更好地理解肿瘤的进展。
