基于金纳米粒子修饰的二硫化钼纳米杂化多层纳米探针的 microRNAs 的超灵敏分析。
Ultrasensitive analysis of microRNAs with gold nanoparticle-decorated molybdenum disulfide nanohybrid-based multilayer nanoprobes.
机构信息
Key Laboratory for Organic Electronics and Information Displays (KLOEID) & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.
出版信息
Chem Commun (Camb). 2020 Aug 14;56(63):9012-9015. doi: 10.1039/d0cc03845h. Epub 2020 Jul 8.
The nanoprobe-based signal amplification strategy is a powerful way to ultrasensitively detect biomolecules. Herein, a gold nanoparticle-decorated molybdenum disulfide (MoS-AuNP)-based multilayer nanoprobe (MLNP) was designed for ultrasensitive analysis of microRNA-21 (miRNA-21). The MLNP-amplified electrochemical biosensor exhibited an ultrawide dynamic range (10 aM-1 μM) and an ultralow detection limit (38 aM) for target miRNA-21 analysis. Furthermore, this biosensor can determine miRNA-21 expression in cell lysates of 100 human cervical cancer (HeLa) cells. Our results demonstrate that MoS-AuNP nanocomposites have great potential in constructing biosensors for target molecule analysis.
基于纳米探针的信号放大策略是超灵敏检测生物分子的一种强大方法。在此，设计了一种基于金纳米粒子修饰的二硫化钼（MoS-AuNP）的多层纳米探针（MLNP），用于超灵敏分析 microRNA-21（miRNA-21）。MLNP 放大的电化学生物传感器对靶 miRNA-21 的分析表现出超宽的动态范围（10 aM-1 μM）和超低的检测限（38 aM）。此外，该生物传感器可以测定 100 个人宫颈癌细胞（HeLa）细胞裂解液中的 miRNA-21 表达。我们的结果表明，MoS-AuNP 纳米复合材料在构建用于目标分子分析的生物传感器方面具有巨大的潜力。
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