Department of Biochemistry and Molecular Genetics, College of Graduate Studies, Midwestern University, Downers Grove, IL, USA.
Biomedical Sciences Program, College of Graduate Studies, Midwestern University, Downers Grove, IL, USA.
Oral Dis. 2021 Mar;27(2):215-225. doi: 10.1111/odi.13542. Epub 2020 Aug 2.
To compare the effects of dietary fat and sex on murine oral squamous cell carcinoma pathology.
Male and female C57Bl/6 mice (36/sex) received a low-fat (10 kcal%) or high-fat (60 kcal%) diet. Water (control), vehicle, or 4-nitroquinoline-1-oxide in vehicle (50 μg/ml) was provided for 17 weeks followed by six additional weeks of water. Oral lesion development was recorded weekly. Histopathologic changes in tongues were examined, and T cells (CD3+), macrophages (CD68+), and neutrophils (Ly6+) were quantified.
All 4-nitroquinoline-1-oxide-treated mice developed oral tumors. High-fat diet exacerbated pathology, demonstrated by an increased final tumor burden (10.9 ± 4.5 vs. 7.9 ± 2.5, mm/mouse, p < .05; high-fat diet vs. low-fat diet, respectively), and a greater histopathology score. When dietary groups were combined, 4-nitroquinoline-1-oxide-treated males displayed higher histopathology scores than females (4.2 ± 0.3 vs. 3.6 ± 0.2, respectively, p < .05). Lymphoid cell infiltration was greater in the 4-nitroquinoline-1-oxide mouse tongues than controls: T cells (14.0 vs. 0.96 cells/mm ), macrophages (3.6 vs. 1.8 cells/mm ), and neutrophils (12.0 vs. 0.38 cells/mm ).
High-fat diet and male sex increased the pathology of 4-nitroquinoline-1-oxide-induced oral cancer. Elevated lymphoid cell infiltration contributed to disease pathology.
比较饮食脂肪和性别对小鼠口腔鳞状细胞癌病理的影响。
雄性和雌性 C57Bl/6 小鼠（每组 36 只）分别给予低脂（10%热量）或高脂（60%热量）饮食。给予水（对照）、载体或载体中的 4-硝基喹啉-1-氧化物（50μg/ml）17 周，然后再给予水 6 周。每周记录口腔病变的发展情况。检查舌部的组织病理学变化，并对 T 细胞（CD3+）、巨噬细胞（CD68+）和中性粒细胞（Ly6+）进行定量分析。
所有接受 4-硝基喹啉-1-氧化物处理的小鼠均发展出口腔肿瘤。高脂饮食加剧了病理变化，表现为最终肿瘤负担增加（10.9±4.5 与 7.9±2.5，mm/只，p<.05；分别为高脂饮食与低脂饮食）和组织病理学评分增加。当将饮食组合并时，接受 4-硝基喹啉-1-氧化物处理的雄性小鼠的组织病理学评分高于雌性（4.2±0.3 与 3.6±0.2，分别为 p<.05）。与对照组相比，4-硝基喹啉-1-氧化物处理的小鼠舌部的淋巴细胞浸润更多：T 细胞（14.0 与 0.96 个细胞/mm）、巨噬细胞（3.6 与 1.8 个细胞/mm）和中性粒细胞（12.0 与 0.38 个细胞/mm）。
高脂饮食和雄性性别增加了 4-硝基喹啉-1-氧化物诱导的口腔癌的病理变化。淋巴细胞浸润的增加导致了疾病的病理学变化。
