Cantrell Maranda S, Seale Jared T, Arispe Sergio A, McDougal Owen M
Biomolecular Sciences Ph.D. Program, Boise State University, Boise, ID 83725, USA.
Department of Chemistry and Biochemistry, Boise State University, Boise, ID 83725, USA.
Foods. 2020 Jul 6;9(7):884. doi: 10.3390/foods9070884.
Qualitative and semi-quantitative analysis of organosulfides extracted from oil obtained by steam distillation of yellow onions was performed by gas chromatography-mass spectrometry (GC-MS). The extraction efficiency of organosulfides from onion oil was evaluated across four solvents: dichloromethane; diethyl ether; n-pentane; and hexanes. Analysis of solvent extracted organosulfides by GC-MS provided qualitative results that support the use of dichloromethane over other solvents based on identification of 27 organosulfides from the dichloromethane extract as compared to 10 from diethyl ether; 19 from n-pentane; and 17 from hexanes. Semi-quantitative evaluation of organosulfides present in the dichloromethane extract was performed using diallyl disulfide as the internal reference standard. Three organosulfides were detected in the extract at ≥5 mg/kg; 18 organosulfides between 3-5 mg/kg; and six organosulfides at <3 mg/kg. The E/Z isomers of 1-propenyl propyl trisulfide were among the most prevalent components extracted from the onion oil across all solvents; and 3,6-diethyl-1,2,4,5-tetrathiane was among the most abundant organosulfides in all solvents except hexanes. The method described here for the extraction of organosulfides from steam distilled onion oil surveys common solvents to arrive at a qualitative and semi-quantitative method of analysis for agricultural products involving onions; onion oil; and secondary metabolites of spp.
采用气相色谱-质谱联用仪（GC-MS）对通过蒸汽蒸馏黄洋葱获得的油中提取的有机硫化物进行定性和半定量分析。在四种溶剂中评估了从洋葱油中提取有机硫化物的效率：二氯甲烷、乙醚、正戊烷和己烷。通过GC-MS对溶剂萃取的有机硫化物进行分析，得到的定性结果表明，与从乙醚中鉴定出10种、正戊烷中鉴定出19种、己烷中鉴定出17种有机硫化物相比，基于从二氯甲烷提取物中鉴定出27种有机硫化物，二氯甲烷比其他溶剂更适合使用。以二烯丙基二硫化物为内标物，对二氯甲烷提取物中存在的有机硫化物进行半定量评估。在提取物中检测到三种有机硫化物含量≥5 mg/kg；18种有机硫化物含量在3 - 5 mg/kg之间；六种有机硫化物含量<3 mg/kg。在所有溶剂中，1-丙烯基丙基三硫化物的E/Z异构体是从洋葱油中提取的最普遍成分之一；在除己烷外的所有溶剂中，3,6-二乙基-1,2,4,5-四硫杂环己烷是含量最丰富的有机硫化物之一。本文所述的从蒸汽蒸馏洋葱油中提取有机硫化物的方法，对常用溶剂进行了研究，以得出一种针对涉及洋葱、洋葱油和 spp. 次级代谢产物的农产品的定性和半定量分析方法。
