Yatham Priyanka, Shukla Devendra, Srivastava Amit K, Pragadheesh V S, Kumar Deepak
Organic and Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology (IICB), Kolkata, West Bengal, India.
National Institute of Pharmaceutical Education and Research (NIPER), Kolkata, West Bengal, India.
Nat Prod Res. 2022 Jun;36(11):2869-2874. doi: 10.1080/14786419.2021.1922903. Epub 2021 May 7.
, containing organosulfides is widely used in Indian cuisine and traditionally claimed to have several medicinal properties including anticancer properties. enriched with organosulfides displayed significant inhibition of the cell growth against SKOV3 and A549 cancer cells in a dose-dependent manner. This prompted us to investigate and delineate the compounds responsible for the activity. In this endeavor, the employed GC/GC-MS analysis resulted in the indecisive outcome. This led to the development of an expedient isocratic RP-HPLC method for the separation and purification of four major compounds which were further unambiguously characterised as (-)--2-butyl propenyl disulfide, (-)--2-butyl propenyl disulfide, (-)-1-(methylthio)propyl()-1-propenyl disulfide, and (-)-1-(methylthio)propyl()-1-propenyl disulfide employing H, C, and 2 D NMR. The isolated compounds were further evaluated for their potential against SKOV3 and A549 cell lines where a trisulfide has displayed better activity.
含有有机硫化物的[具体物质未提及]在印度菜肴中广泛使用，传统上声称具有多种药用特性，包括抗癌特性。富含有机硫化物的[具体物质未提及]对SKOV3和A549癌细胞的细胞生长具有显著的剂量依赖性抑制作用。这促使我们研究并确定具有该活性的化合物。在这项工作中，所采用的气相色谱/气相色谱 - 质谱分析结果不明确。这导致开发了一种便捷的等度反相高效液相色谱方法，用于分离和纯化四种主要化合物，这些化合物通过氢谱、碳谱和二维核磁共振进一步明确表征为(-)--2-丁基丙烯基二硫化物、(-)--2-丁基丙烯基二硫化物、(-)-1-(甲硫基)丙基()-1-丙烯基二硫化物和(-)-1-(甲硫基)丙基()-1-丙烯基二硫化物。对分离出的化合物针对SKOV3和A549细胞系的潜力进行了进一步评估，其中一种三硫化物显示出更好的活性。
