Seppälä I, Sarvas H, Mäkelä O, Mattila P, Eskola J, Käyhty H
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Scand J Immunol. 1988 Oct;28(4):471-9. doi: 10.1111/j.1365-3083.1988.tb01478.x.
Antigenicity of two Haemophilus influenzae type b (Hib) conjugate vaccines was studied by immunizing adults and 2-year-old children. Both vaccines induced strong anti-Hib responses and strong antibody responses to diphtheria toxin (DT), the protein part of the conjugate. The adults' responses were stronger than the children's. A conjugate of Hib oligosaccharide and mutant diphtheria toxin (HbOC) emerged as slightly superior to a conjugate of Hib polysaccharide and diphtheria toxoid (PRP-D). HbOC induced somewhat higher total anti-Hib responses and significantly higher IgG1 anti-Hib responses than PRP-D. IgG1 and IgG2 were the main IgG subclasses of the anti-Hib antibodies, whereas IgG1 and IgG4 were the main subclasses of the anti-DT antibodies. Within this main rule, the ratio IgG1/IgG2 of anti-Hib antibodies varied between individuals. The average ratio was higher than five in children but approximately one in adults. It was lower in adult recipients of the polysaccharide conjugate (0.69) than in adult recipients of the oligosaccharide conjugate (1.55). A large interindividual variation was observed in concentrations of IgG2 of Hib specificity, perhaps reflecting a small number of IgG2-committed B-cell clones participating in the response.
通过对成人和2岁儿童进行免疫接种，研究了两种b型流感嗜血杆菌（Hib）结合疫苗的抗原性。两种疫苗均诱导了强烈的抗Hib反应以及对白喉毒素（DT，结合物的蛋白质部分）的强烈抗体反应。成人的反应比儿童更强。Hib寡糖与突变白喉毒素的结合物（HbOC）表现出略优于Hib多糖与白喉类毒素的结合物（PRP-D）。与PRP-D相比，HbOC诱导的总抗Hib反应略高，IgG1抗Hib反应显著更高。IgG1和IgG2是抗Hib抗体的主要IgG亚类，而IgG1和IgG4是抗DT抗体的主要亚类。在这一主要规律范围内，抗Hib抗体的IgG1/IgG2比值在个体间有所不同。儿童的平均比值高于5，而成人的平均比值约为1。多糖结合物的成年接种者（0.69）的该比值低于寡糖结合物的成年接种者（1.55）。观察到Hib特异性IgG2浓度存在较大的个体间差异，这可能反映了参与反应的IgG2定向B细胞克隆数量较少。
