Fauziah Riska Rian, Ogita Shinjiro, Yoshino Tomoyuki, Yamamoto Yukihiro
Faculty of Life and Environmental Science, Prefectural University of Hiroshima.
Faculty of Agricultural Technology, University of Jember.
J Oleo Sci. 2020 Aug 6;69(8):801-807. doi: 10.5650/jos.ess20028. Epub 2020 Jul 9.
The health benefits of conjugated linoleic acid (CLA), a functional lipid with anti-cancer, anti-obesity, and hypotensive activity, have garnered increasing attention. The current study was conducted to determine the oxidative stability of CLA in the form of triacylglycerol (CLA-TAG) and phosphatidylcholine (CLA-PC) at the sn-2 position. Oxidation was performed at 30°C or 40°C in the dark. Hydroperoxides, as the primary oxidation products, were analyzed using diphenyl-1-pyrenylphosphine. Thiobarbituric acid reactive substances (TBARS) and volatile compounds were monitored as secondary oxidation products. The results suggest that CLA-PC was more stable against oxidation than CLA-TAG from the perspective of suppression of the generation of hydroperoxides and TBARS. However, CLA-PC produced more volatile compounds than CLA-TAG. We suggest that choline was released during the oxidation of CLA-PC, and acted as an antioxidant. The ensuing reaction between choline and hydroperoxide induced the generation of volatile compounds such as pentanal, hexanal, and heptanal.
共轭亚油酸（CLA）是一种具有抗癌、抗肥胖和降血压活性的功能性脂质，其对健康的益处已受到越来越多的关注。本研究旨在确定处于sn-2位的三酰甘油形式（CLA-TAG）和磷脂酰胆碱形式（CLA-PC）的CLA的氧化稳定性。氧化在30°C或40°C黑暗条件下进行。使用二苯基-1-芘基膦分析作为主要氧化产物的氢过氧化物。监测硫代巴比妥酸反应性物质（TBARS）和挥发性化合物作为次要氧化产物。结果表明，从抑制氢过氧化物和TBARS生成的角度来看，CLA-PC比CLA-TAG对氧化更稳定。然而，CLA-PC比CLA-TAG产生更多的挥发性化合物。我们认为胆碱在CLA-PC氧化过程中释放，并起到抗氧化剂的作用。随后胆碱与氢过氧化物之间的反应诱导了戊醛、己醛和庚醛等挥发性化合物的生成。
