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淋巴细胞激活过程中一种溶酶体酶的超微结构研究。

Ultrastructural studies of a lysosomal enzyme during lymphocyte activation.

作者信息

Bou-Gharios G, Moss J, Abraham D, Partridge T, Olsen I

机构信息

Department of Histopathology, Charing Cross and Westminster Medical School, London, UK.

出版信息

Br J Exp Pathol. 1988 Oct;69(5):661-70.

PMID:3264180
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2013276/
Abstract

A post-embedding immunogold technique has been used for the ultrastructural localization of a lysosomal enzyme, beta-glucuronidase, in resting and activated T- and B-lymphocytes. The results presented here show that mitogen-induced stimulation of T- and B-cells was associated with an increase in the amount of enzyme in the Golgi complex and rough endoplasmic reticulum, organelles which were rarely present in the resting lymphocytes.

摘要

一种包埋后免疫金技术已被用于在静止和活化的T淋巴细胞和B淋巴细胞中对溶酶体酶β-葡萄糖醛酸酶进行超微结构定位。此处呈现的结果表明,丝裂原诱导的T细胞和B细胞刺激与高尔基体和粗面内质网中酶量的增加相关,而这些细胞器在静止淋巴细胞中很少出现。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fd2/2013276/d782f0694bc0/brjexppathol00005-0066-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fd2/2013276/48efd736e649/brjexppathol00005-0062-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fd2/2013276/22e4e84f6ac1/brjexppathol00005-0064-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fd2/2013276/7ba424cc5fca/brjexppathol00005-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fd2/2013276/d782f0694bc0/brjexppathol00005-0066-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fd2/2013276/48efd736e649/brjexppathol00005-0062-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fd2/2013276/22e4e84f6ac1/brjexppathol00005-0064-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fd2/2013276/7ba424cc5fca/brjexppathol00005-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fd2/2013276/d782f0694bc0/brjexppathol00005-0066-a.jpg

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Ultrastructural studies of a lysosomal enzyme during lymphocyte activation.淋巴细胞激活过程中一种溶酶体酶的超微结构研究。
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引用本文的文献

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Lysosomal storage diseases: mechanisms of enzyme replacement therapy.溶酶体贮积症:酶替代疗法的机制
Histochem J. 1993 Sep;25(9):593-605. doi: 10.1007/BF00157873.
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Localization of lysosomal antigens in activated T-lymphocytes.溶酶体抗原在活化T淋巴细胞中的定位
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本文引用的文献

1
LYSOSOMES IN DIVIDING CELLS, WITH SPECIAL REFERENCE TO LYMPHOCYTES.分裂细胞中的溶酶体,特别涉及淋巴细胞
Lancet. 1964 Dec 26;2(7374):1371-3. doi: 10.1016/s0140-6736(64)91162-6.
2
Acquisition of beta-glucuronidase activity by deficient fibroblasts during direct contact with lymphoid cells.在与淋巴细胞直接接触期间,缺陷型成纤维细胞获得β-葡萄糖醛酸酶活性。
J Cell Sci. 1982 Jun;55:211-31. doi: 10.1242/jcs.55.1.211.
3
Lysosomal acid phosphatase: activity and isoenzymes in separated normal human blood cells.溶酶体酸性磷酸酶:正常人分离血细胞中的活性与同工酶
Clin Chim Acta. 1980 Mar 28;102(2-3):227-35. doi: 10.1016/0009-8981(80)90037-6.
4
Acid phosphatase in peripheral blood lymphocyte lysosomes in patients with diabetes mellitus.糖尿病患者外周血淋巴细胞溶酶体中的酸性磷酸酶
Folia Histochem Cytochem (Krakow). 1983;21(1):15-22.
5
Studies on the activities and properties of lysosomal hydrolases in fractionated populations of human peripheral blood cells.人类外周血细胞分离群体中溶酶体水解酶的活性和特性研究。
Clin Chim Acta. 1980 Feb 14;101(1):33-44. doi: 10.1016/0009-8981(80)90053-4.
6
Immunocytochemical localization of beta-glucuronidase in the male rat preputial gland.雄性大鼠包皮腺中β-葡萄糖醛酸酶的免疫细胞化学定位
Eur J Cell Biol. 1982 Jun;27(2):213-20.
7
Lysosomal glycohydrolases in normal T and non-T peripheral lymphocytes.
Clin Chim Acta. 1984 Feb 14;137(1):67-75. doi: 10.1016/0009-8981(84)90313-9.
8
Arylsulfatase in natural killer cells: its possible role in cytotoxicity.自然杀伤细胞中的芳基硫酸酯酶:其在细胞毒性中的可能作用。
Proc Natl Acad Sci U S A. 1983 Nov;80(22):6977-81. doi: 10.1073/pnas.80.22.6977.
9
Effects of mitogenic stimulation of lymphocytes on lysosomal enzyme activity.有丝分裂原刺激淋巴细胞对溶酶体酶活性的影响。
Cell Biochem Funct. 1983 Jul;1(2):103-8. doi: 10.1002/cbf.290010211.
10
Correlation of lysosomal enzyme abnormalities in various forms of adult leukaemia.成人白血病各种类型中溶酶体酶异常的相关性
J Clin Pathol. 1983 Sep;36(9):1000-4. doi: 10.1136/jcp.36.9.1000.