Besley G T, Moss S E, Bain A D, Dewar A E
J Clin Pathol. 1983 Sep;36(9):1000-4. doi: 10.1136/jcp.36.9.1000.
Lysosomal enzyme activities were studied in cells derived from the following types of leukaemia: chronic myeloid, acute myeloid, acute myelomonocytic, acute monocytic, non-T, non-B cell acute lymphoblastic, T-cell acute lymphoblastic, B-cell chronic lymphocytic and T-cell chronic lymphocytic. Activities of beta-hexosaminidase and alpha-mannosidase were significantly higher in cells from acute monocytic and acute myelomonocytic leukaemias, and somewhat higher in the other myeloid leukaemias, when compared with control granulocytes. Activities of beta-hexosaminidase, alpha-mannosidase, alpha-fucosidase, beta-glucuronidase and acid phosphatase were markedly lower in B cells of chronic lymphocytic leukaemia when compared with control or other leukaemic lymphoid cells. On isoelectric focusing abnormal patterns of beta-hexosaminidase, alpha-mannosidase and beta-glucuronidase activities were commonly found in myeloid and non-T, non-B cell leukaemias. All patients with acute myeloid leukaemia exhibited a relative decrease in the B form of beta-hexosaminidase activity. The results described show that studies on lysosomal enzymes may assist in the classification of different types of leukaemia.
慢性粒细胞白血病、急性髓细胞白血病、急性粒单核细胞白血病、急性单核细胞白血病、非T非B细胞急性淋巴细胞白血病、T细胞急性淋巴细胞白血病、B细胞慢性淋巴细胞白血病和T细胞慢性淋巴细胞白血病。与对照粒细胞相比，急性单核细胞白血病和急性粒单核细胞白血病细胞中的β-己糖胺酶和α-甘露糖苷酶活性显著更高，其他髓细胞白血病中的这两种酶活性则略高。与对照或其他白血病淋巴细胞相比，慢性淋巴细胞白血病B细胞中的β-己糖胺酶、α-甘露糖苷酶、α-岩藻糖苷酶、β-葡萄糖醛酸酶和酸性磷酸酶活性明显更低。在等电聚焦时，髓细胞白血病以及非T非B细胞白血病中常见β-己糖胺酶、α-甘露糖苷酶和β-葡萄糖醛酸酶活性的异常模式。所有急性髓细胞白血病患者的β-己糖胺酶活性的B型均相对降低。所述结果表明，对溶酶体酶的研究可能有助于不同类型白血病的分类。
